1887

Abstract

The fungal pathogen has caused declines and extinctions in hundreds of amphibian species across the world. Virulence varies among and within lineages; the Global Panzootic Lineage (GPL) is the most pathogenic, although there is also variation in lethality among GPL isolates. Amphibians have a number of defences against pathogens, and skin products including the microbiota and host peptides have considerable influence over disease progression. Here we demonstrate that the collective skin products (the mucosome) of two amphibian species show significant variation in their ability to inhibit different globally distributed isolates of GPL. This may in part explain the variation in disease susceptibility of hosts to different strains of . More work is required to identify particular traits associated with mucosomes that confer broad-spectrum inhibition across GPL in order to facilitate the development of prophylaxis and/or treatments for chytridiomycosis .

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/content/journal/micro/10.1099/mic.0.000570
2017-12-01
2024-12-12
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References

  1. Berger L, Roberts AA, Voyles J, Longcore JE, Murray KA et al. History and recent progress on chytridiomycosis in amphibians. Fungal Ecol 2016; 19:89–99 [View Article]
    [Google Scholar]
  2. Longcore JE, Pessier AP, Nichols DK. Batrachochytrium dendrobatidis gen. et sp. nov., a chytrid pathogenic to amphibians. Mycologia 1999; 91:219–227 [View Article]
    [Google Scholar]
  3. Martel A, Spitzen-van der Sluijs A, Blooi M, Bert W, Ducatelle R et al. Batrachochytrium salamandrivorans sp. nov. causes lethal chytridiomycosis in amphibians. Proc Natl Acad Sci USA 2013; 110:15325–15329 [View Article][PubMed]
    [Google Scholar]
  4. Martel A, Blooi M, Adriaensen C, van Rooij P, Beukema W et al. Wildlife disease. Recent introduction of a chytrid fungus endangers Western Palearctic salamanders. Science 2014; 346:630–631 [View Article][PubMed]
    [Google Scholar]
  5. van Rooij P, Martel A, Haesebrouck F, Pasmans F. Amphibian chytridiomycosis: a review with focus on fungus-host interactions. Vet Res 2015; 46:137 [View Article][PubMed]
    [Google Scholar]
  6. Berger L, Marantelli G, Skerratt LF, Speare R. Virulence of the amphibian chytrid fungus Batrachochytium dendrobatidis varies with the strain. Dis Aquat Organ 2005; 68:47–50 [View Article][PubMed]
    [Google Scholar]
  7. Fisher MC, Bosch J, Yin Z, Stead DA, Walker J et al. Proteomic and phenotypic profiling of the amphibian pathogen Batrachochytrium dendrobatidis shows that genotype is linked to virulence. Mol Ecol 2009; 18:415–429 [View Article][PubMed]
    [Google Scholar]
  8. Farrer RA, Weinert LA, Bielby J, Garner TW, Balloux F et al. Multiple emergences of genetically diverse amphibian-infecting chytrids include a globalized hypervirulent recombinant lineage. Proc Natl Acad Sci USA 2011; 108:18732–18736 [View Article][PubMed]
    [Google Scholar]
  9. Doddington BJ, Bosch J, Oliver JA, Grassly NC, Garcia G et al. Context-dependent amphibian host population response to an invading pathogen. Ecology 2013; 94:1795–1804 [View Article][PubMed]
    [Google Scholar]
  10. Woodhams DC, Brandt H, Baumgartner S, Kielgast J, Küpfer E et al. Interacting symbionts and immunity in the amphibian skin mucosome predict disease risk and probiotic effectiveness. PLoS One 2014; 9:e96375 [View Article][PubMed]
    [Google Scholar]
  11. Antwis RE, Preziosi RF, Harrison XA, Garner TW. Amphibian symbiotic bacteria do not show a universal ability To inhibit growth of the global panzootic lineage of Batrachochytrium dendrobatidis. Appl Environ Microbiol 2015; 81:3706–3711 [View Article][PubMed]
    [Google Scholar]
  12. Muletz-Wolz CR, Almario JG, Barnett SE, Direnzo GV, Martel A et al. Inhibition of fungal pathogens across genotypes and temperatures by amphibian skin bacteria. Front Microbiol 2017; 8:1551 [View Article][PubMed]
    [Google Scholar]
  13. Bletz MC, Myers J, Woodhams DC, Rabemananjara FCE, Rakotonirina A et al. Estimating Herd Immunity to Amphibian Chytridiomycosis in Madagascar based on the defensive function of amphibian skin bacteria. Front Microbiol 2017; 8:8 [View Article][PubMed]
    [Google Scholar]
  14. Bell SC, Alford RA, Garland S, Padilla G, Thomas AD. Screening bacterial metabolites for inhibitory effects against Batrachochytrium dendrobatidis using a spectrophotometric assay. Dis Aquat Organ 2013; 103:77–85 [View Article][PubMed]
    [Google Scholar]
  15. Becker MH, Walke JB, Murrill L, Woodhams DC, Reinert LK et al. Phylogenetic distribution of symbiotic bacteria from Panamanian amphibians that inhibit growth of the lethal fungal pathogen Batrachochytrium dendrobatidis. Mol Ecol 2015; 24:1628–1641 [View Article][PubMed]
    [Google Scholar]
  16. Jani AJ, Briggs CJ. The pathogen Batrachochytrium dendrobatidis disturbs the frog skin microbiome during a natural epidemic and experimental infection. Proc Natl Acad Sci USA 2014; 111:E5049E5058 [View Article][PubMed]
    [Google Scholar]
  17. Becker MH, Walke JB, Cikanek S, Savage AE, Mattheus N et al. Composition of symbiotic bacteria predicts survival in Panamanian golden frogs infected with a lethal fungus. Proc Biol Sci 2015; 282:20142881 [View Article][PubMed]
    [Google Scholar]
  18. Rebollar EA, Hughey MC, Medina D, Harris RN, Ibáñez R et al. Skin bacterial diversity of Panamanian frogs is associated with host susceptibility and presence of Batrachochytrium dendrobatidis. Isme J 2016; 10:1682–1695 [View Article][PubMed]
    [Google Scholar]
  19. Walke JB, Becker MH, Loftus SC, House LL, Teotonio TL et al. Community structure and function of amphibian skin microbes: an experiment with bullfrogs exposed to a chytrid fungus. PLoS One 2015; 10:e0139848 [View Article][PubMed]
    [Google Scholar]
  20. Farrer RA, Henk DA, Garner TW, Balloux F, Woodhams DC et al. Chromosomal copy number variation, selection and uneven rates of recombination reveal cryptic genome diversity linked to pathogenicity. PLoS Genet 2013; 9:e1003703 [View Article][PubMed]
    [Google Scholar]
  21. Tarrant J, Cilliers D, Du Preez LH, Weldon C. Spatial assessment of amphibian chytrid fungus (Batrachochytrium dendrobatidis) in South Africa confirms endemic and widespread infection. PLoS One 2013; 8:e69591 [View Article][PubMed]
    [Google Scholar]
  22. Garner TW, Schmidt BR, Martel A, Pasmans F, Muths E et al. Mitigating amphibian chytridiomycoses in nature. Philos Trans R Soc Lond B Biol Sci 2016; 371:20160207 [View Article][PubMed]
    [Google Scholar]
  23. Bletz MC, Loudon AH, Becker MH, Bell SC, Woodhams DC et al. Mitigating amphibian chytridiomycosis with bioaugmentation: characteristics of effective probiotics and strategies for their selection and use. Ecol Lett 2013; 16:807–820 [View Article][PubMed]
    [Google Scholar]
  24. Rebollar EA, Antwis RE, Becker MH, Belden LK, Bletz MC et al. Using "Omics" and integrated multi-omics approaches to guide probiotic selection to mitigate chytridiomycosis and other emerging infectious diseases. Front Microbiol 2016; 7:68 [View Article][PubMed]
    [Google Scholar]
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