RT Journal Article SR Electronic(1) A1 Kouril, Theresa A1 Eicher, Johann J. A1 Siebers, Bettina A1 Snoep, Jacky L.YR 2017 T1 Phosphoglycerate kinase acts as a futile cycle at high temperature JF Microbiology, VO 163 IS 11 SP 1604 OP 1612 DO https://doi.org/10.1099/mic.0.000542 PB Microbiology Society, SN 1465-2080, AB In (hyper)thermophilic organisms metabolic processes have to be adapted to function optimally at high temperature. We compared the gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate at 30 °C and at 70 °C. At 30 °C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 °C, 1,3-bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. When phosphoglycerate kinase was incubated together with glyceraldehyde 3-phosphate dehydrogenase it was possible to convert 3-phosphoglycerate to glyceraldehyde 3-phosphate, both at 30 °C and at 70 °C, however, at 70 °C only low concentrations of product were observed due to thermal instability of glyceraldehyde 3-phosphate. Thus, thermolabile intermediates challenge central metabolic reactions and require special adaptation strategies for life at high temperature., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000542