1887

Abstract

Daptomycin is a lipopeptide antibiotic with activity against Gram-positive bacteria. We showed previously that can survive daptomycin exposure by releasing membrane phospholipids that inactivate the antibiotic. To determine whether other pathogens possess this defence mechanism, phospholipid release and daptomycin activity were measured after incubation of , group A or B streptococci, or with the antibiotic. All bacteria released phospholipids in response to daptomycin, which resulted in at least partial inactivation of the antibiotic. However, showed the highest levels of lipid release and daptomycin inactivation. As shown previously for , phospholipid release by was inhibited by the lipid biosynthesis inhibitor platensimycin. In conclusion, several pathogenic Gram-positive bacteria, including inactivate daptomycin by releasing phospholipids, which may contribute to the failure of daptomycin to resolve infections caused by these pathogens.

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2017-10-01
2024-12-05
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