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Volume 163, Issue 9

Involvement of signal peptidase I in biofilm formation Open Access

Abstract

Biofilm accounts for 65–80 % of microbial infections in humans. Considerable evidence links biofilm formation by oral microbiota to oral disease and consequently systemic infections. , a Gram-positive bacterium, is one of the most abundant species of the oral microbiota and it contributes to biofilm development in the oral cavity. Due to its altered biofilm formation, we investigated a biofilm mutant, Δ, that is deficient in type I signal peptidase (SPase) in this study. Although the growth curve of the Δ mutant showed no significant difference from that of the wild-type strain SK36, biofilm assays using both microtitre plate assay and confocal laser scanning microscopy (CLSM) confirmed a sharp reduction in biofilm formation in the mutant compared to the wild-type strain and the paralogous mutant ΔSSA_0849. Scanning electron microscopy (SEM) revealed remarkable differences in the cell surface morphologies and chain length of the Δ mutant compared with those of the wild-type strain. Transcriptomic and proteomic assays using RNA sequencing and mass spectrometry, respectively, were conducted on the Δ mutant to evaluate the functional impact of SPase on biofilm formation. Subsequently, bioinformatics analysis revealed a number of proteins that were differentially regulated in the Δ mutant, narrowing down the list of SPase substrates involved in biofilm formation to lactate dehydrogenase (SSA_1221) and a short-chain dehydrogenase (SSA_0291). With further experimentation, this list defined the link between -encoded SPase, cell wall biosynthesis and biofilm formation.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , mass spectrometry , RNA-seq , signal peptidase and Streptococcus sanguinis
© 2017 The Authors
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2017-09-01
2024-04-19
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Involvement of signal peptidase I in Streptococcus sanguinis biofilm formation
Microbiology 163, 1306 (2017); https://doi.org/10.1099/mic.0.000516
/content/journal/micro/10.1099/mic.0.000516
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