1887

Abstract

sp. A2 is a novel Gram-negative sulfate-reducing bacterium that was isolated from sediments of the Norilsk mining/smelting area in Russia. The organism possesses a monocistronic operon encoding a 71 kDa periplasmic multicopperoxidase, which we call DA2_CueO. Histidine-tagged DA2_CueO expressed from a plasmid in and purified by Ni–NTA affinity chromatography oxidizes Cu and Fe, and exhibits phenol oxidase activity with 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), 2,3-dihydroxybenzoic acid and 2,6-dimethoxyphenol as substrates, using O as the oxidant. When expressed in an knock-out strain, DA2_CueO exhibits phenol oxidase activity and enhances the copper tolerance of the strain. These findings indicate that the gene of sp. A2 encodes a multicopperoxidase with a role in metal ion resistance. The enzyme displays some novel structural features, which are discussed.

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2017-08-01
2020-01-27
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