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Volume 163, Issue 7

Molecular and biochemical characteristics of the inulosucrase HugO from DSM40736 (Tü494) Free

Abstract

Polyfructans are synthesized from sucrose by plants (mostly inulin) and by both Gram-negative and Gram-positive bacteria (mostly levan). In the phylum only levan synthesis by species has been reported. We have identified a putative fructansucrase gene () in DSM40736 (Tü494). HugO was heterologously expressed and biochemically characterized. HPSEC-MALLS and 2D-H-C nuclear magnetic resonance (NMR) spectroscopy analysis showed that the fructan polymer produced has an Molecular Weight of 2.5*10 Da and is an inulin that is mainly composed of (β2–1)-linked fructose units. This is the first report of a fructansucrase from and an inulosucrase from . Database searches showed that fructansucrases clearly occur more widely in streptomycetes. Analysis of the active site of HugO and other actinobacterial Gram-positive fructansucrases revealed that their +1 substrate-binding sites are conserved, but are most similar to those in Gram-negative fructansucrases. HugO also resembles Gram-negative fructansucrases in not requiring calcium ions for activity. The origin and properties of HugO and other actinobacterial fructansucrases thus clearly differ from those of previously characterized Gram-positive fructansucrases.

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Keyword(s): fructan , fructooligosaccharides , GH68 , inulosucrase and Streptomyces viridochromogenes
© 2017 The Authors
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2017-07-01
2024-04-24
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Molecular and biochemical characteristics of the inulosucrase HugO from Streptomyces viridochromogenes DSM40736 (Tü494)
Microbiology 163, 1030 (2017); https://doi.org/10.1099/mic.0.000493
/content/journal/micro/10.1099/mic.0.000493
/content/journal/micro/10.1099/mic.0.000493
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