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Volume 163, Issue 5

Impact of temperature on SP17 morphology and biofilm structure during growth on alkanes Free

Abstract

Alkanes are widespread pollutants found in soil, freshwater and marine environments. () strain SP17 is a marine bacterium able to use many hydrophobic organic compounds, including alkanes, through the production of biofilms that allow their poor solubility to be overcome. This study pointed out that temperature is an environmental factor that strongly affects the biofilm formation and morphology of on the model alkanes, hexadecane and paraffin. We showed that biofilm formation and accumulation of intracytoplasmic inclusions are higher on solid alkanes (hexadecane at 10 °C and paraffin at 10 °C and 30 °C) than on liquid alkane (hexadecane at 30 °C) or soluble substrate (lactate at both temperatures). We also found that produces more extracellular polymeric substances at 30 °C than at 10 °C on alkanes and none on lactate. We observed that bacterial length is significantly higher at 10 °C than at 30 °C on lactate and hexadecane. On paraffin, at 30 °C, the cell morphology is markedly altered by large rounded or irregularly shaped cytoplasmic inclusions. Altogether, the results showed that is able to adapt and use alkanes as a carbon source, even at low temperature.

  • Received:
  • Accepted:
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Keyword(s): biofilm , cell shape , lipid inclusion , Marinobacter , physical state of the substrate/substratum , poorly soluble substrate and temperature
© 2017 The Authors
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2017-05-01
2024-04-19
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Impact of temperature on Marinobacter hydrocarbonoclasticus SP17 morphology and biofilm structure during growth on alkanes
Microbiology 163, 669 (2017); https://doi.org/10.1099/mic.0.000466
/content/journal/micro/10.1099/mic.0.000466
/content/journal/micro/10.1099/mic.0.000466
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