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Volume 163, Issue 4

Genome amplification and promoter mutation expand the range of -dependent biofilm responses in an STEC population Free

Abstract

Expression of the major biofilm components of , curli fimbriae and cellulose, requires the CsgD transcription factor. A complex regulatory network allows environmental control of transcription and biofilm formation. However, most clinical serotype O157 : H7 strains contain prophage insertions in the regulator, , or mutations in other regulators that restrict expression. These barriers can be circumvented by certain compensating mutations that restore higher expression. One mechanism is via promoter mutations that switch sigma factor utilization. Biofilm-forming variants utilizing RpoD rather than RpoS have been identified in glycerol freezer stocks of the non-biofilm-forming food-borne outbreak strain, ATCC 43894. In this study we used whole genome sequencing and RNA-seq to study genotypic and transcriptomic differences between those strains. In addition to defining the consequences of the promoter switch and identifying new -controlled genes, we discovered a region of genome amplification in our laboratory stock of 43894 (designated 43894OW) that contributed to the regulation of -dependent properties.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , CsgD , curli , hnr , O157:H7 and STEC
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2017-04-01
2024-04-18
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Genome amplification and promoter mutation expand the range of csgD-dependent biofilm responses in an STEC population
Microbiology 163, 611 (2017); https://doi.org/10.1099/mic.0.000448
/content/journal/micro/10.1099/mic.0.000448
/content/journal/micro/10.1099/mic.0.000448
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