1887

Abstract

The K-12 operon, coding for a predicted oxidoreductase complex, is activated under anaerobic conditions and repressed in the presence of nitrate or nitrite. Anaerobic activation is mediated by the transcription factor FNR, and nitrate/nitrite repression is mediated by NarXL and NarQP. transcription reactions revealed that the DNA upstream of contains sufficient information for RNA polymerase alone to initiate transcription from five locations. FNR severely inhibited synthesis of two of these transcripts (located upstream of, and within, the FNR binding site) and activated the FNR-dependent promoter previously identified . Enhanced expression of in an mutant was consistent with the location of within a promoter island and the FNR-independent transcription observed . FNR-dependent transcription was decreased in the presence of NarL~P. DNaseI footprinting indicated that FNR and NarL~P simultaneously bound at the promoter region and that NarL~P-mediated repression was due to occupation of the 7-2-7 site located downstream of the FNR-dependent promoter. Expression of during the anaerobic growth cycle was repressed when nitrate was present but less so in the presence of nitrite. transcription measurements indicated that the alternative electron acceptors, DMSO and fumarate, could also lower expression, whereas trimethylamine-N-oxide (TMAO) permitted high expression. Therefore, expression of is subject to complex regulation in response to electron acceptor availability that involves at least three transcription factors, FNR (anaerobic activation), NarL~P (nitrate repression) and H-NS (repression in the absence of an antagonist; e.g. FNR).

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2017-04-01
2020-01-17
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