1887

Abstract

To examine the possible physiological significance of Mrp, a multi-subunit cation/proton antiporter from , a chromosomal deletion Δ of was constructed and characterized. The resulting mutant showed a consistent early growth defect in LB broth that became more evident at elevated pH of the growth medium and increasing Na or K loads. After 24 h incubation, these differences disappeared likely due to the concerted effort of other cation pumps in the mutant. Phenotype MicroArray analyses revealed an unexpected systematic defect in nitrogen utilization in the Δ mutant that was complemented by using the operon on an arabinose-inducible expression vector. Deletion of the operon also led to hypermotility, observable on LB and M9 semi-solid agar. Surprisingly, Δ mutation resulted in wild-type biofilm formation in M9 despite a growth defect but the reverse was true in LB. Furthermore, the Δ strain exhibited higher susceptibility to amphiphilic anions. These pleiotropic phenotypes of the Δ mutant demonstrate how the chemiosmotic activity of Mrp contributes to the survival potential of despite the presence of an extended battery of cation/proton antiporters of varying ion selectivity and pH profile operating in the same membrane.

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2016-12-21
2024-12-12
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