%0 Journal Article %A Serata, Masaki %A Kiwaki, Mayumi %A Iino, Tohru %T Functional analysis of a novel hydrogen peroxide resistance gene in Lactobacillus casei strain Shirota %D 2016 %J Microbiology, %V 162 %N 11 %P 1885-1894 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.000379 %K iron-binding %K hydroxyl radical %K hydrogen peroxide %K Fenton reaction %K oxidative stress %K Lactobacillus casei %I Microbiology Society, %X Lactic acid bacteria have a variety of mechanisms for tolerance to oxygen and reactive oxygen species, and these mechanisms differ among species. Lactobacillus casei strain Shirota grows well under aerobic conditions, indicating that the various systems involved in oxidative stress resistance function in this strain. To elucidate the mechanism of oxidative stress resistance in L. casei strain Shirota, we examined the transcriptome response to oxygen or hydrogen peroxide exposure. We then focused on an uncharacterized gene that was found to be up-regulated by both oxygen and hydrogen peroxide stress; we named the gene hprA1 (hydrogen peroxide resistance gene). This gene is widely distributed among lactobacilli. We investigated the involvement of this gene in oxidative stress resistance, as well as the mechanism of tolerance to hydrogen peroxide. Growth of L. casei MS105, an hprA1-disrupted mutant, was not affected by oxygen stress, whereas the survival rate of MS105 after hydrogen peroxide treatment was markedly reduced compared to that of the wild-type. However, the activity of MS105 in eliminating hydrogen peroxide was similar to that of the wild-type. We cloned hprA1 from L. caseiShirota and purified recombinant HprA1 protein from Escherichia coli. We demonstrated that the recombinant HprA1 protein bound to iron and prevented the formation of a hydroxyl radical in vitro. Thus, HprA1 protein probably contributes to hydrogen peroxide tolerance in L. casei strain Shirota by binding to iron in the cells and preventing the formation of a hydroxyl radical. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000379