1887

Abstract

All cells are subjected to oxidative stress, a condition under which reactive oxygen species (ROS) production exceeds elimination. Bacterial defences against ROS include synthesis of antioxidant enzymes like peroxidases and catalases. can produce two distinct catalases, KatB and KatG, which contribute to ROS homeostasis. In this study, we analysed the mechanism behind and expression in two O1 pandemic strains, O395 and N16961, of classical and El Tor biotypes, respectively. Both strains express these genes, especially at stationary phase. However, El Tor N16961 produces higher KatB and KatG levels and is much more resistant to peroxide challenge than the classical strain, confirming a direct relationship between catalase activity and oxidative stress resistance. Moreover, we showed that and expression levels depend on inorganic phosphate (Pi) availability, in contrast to other bacterial species. In N16961, and expression is reduced under Pi limitation relative to Pi abundance. Total catalase activity in N16961 and its mutant cells was similar, independently of growth conditions, indicating that the PhoB/PhoR system is not required for and expression. However, N16961 cells from Pi-limited cultures were 50–100-fold more resistant to HO challenge and accumulated less ROS than mutant cells. Together, these findings suggest that, besides KatB and KatG, the PhoB/PhoR system is an important protective factor against ROS in N16961. They also corroborate previous results from our and other groups, suggesting that the PhoB/PhoR system is fundamental for . biology.

Keyword(s): KatB , KatG and phosphate
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2016-11-23
2019-12-08
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