Expression of alkyl hydroperoxide reductase is regulated negatively by OxyR1 and positively by RpoE2 sigma factor in Sp7 Free

Abstract

OxyR proteins are LysR-type transcriptional regulators, which play an important role in responding to oxidative stress in bacteria. Sp7 harbours two copies of OxyR. The inactivation of the , the gene organized divergently to in Sp7, led to an increased tolerance to alkyl hydroperoxides, which was corroborated by an increase in alkyl hydroperoxide reductase (AhpC) activity, enhanced expression of : fusion and increased synthesis of AhpC protein in the :: mutant. The upstream region of promoter harboured a putative OxyR binding site, T-N11-A. Mutation of T, A or both in the T-N11-Amotif caused derepression of in suggesting that T-N11-A might be the binding site for a negative regulator. Retardation of the electrophoretic mobility of the T-N11-A motif harbouring intergenic DNA by recombinant OxyR1, under reducing as well as oxidizing conditions, indicated that OxyR1 acts as a negative regulator of in . Sequence of the promoter of predicted on the basis of transcriptional start site, and an enhanced expression of fusion in :: mutant background suggested that promoter was RpoE2 dependent. Thus, this study shows that in Sp7, expression is regulated negatively by OxyR1 but is regulated positively by RpoE2, an oxidative-stress-responsive sigma factor. It also shows that OxyR1 regulates the expression RpoE1, which is known to play an important role during photooxidative stress in .

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2016-10-01
2024-03-29
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