1887

Abstract

() and () are major human pathogens known to interact in a variety of disease settings, including airway infections in cystic fibrosis. We recently reported that clinical CF isolates of inhibit the formation and growth of biofilms. Here, we report that the bacteriophage Pf4, produced by , can inhibit the metabolic activity of biofilms. This phage-mediated inhibition was dose dependent, ablated by phage denaturation, and was more pronounced against preformed biofilm rather than biofilm formation. In contrast, planktonic conidial growth was unaffected. Two other phages, Pf1 and fd, did not inhibit , nor did supernatant from a strain incapable of producing Pf4. Pf4, but not Pf1, attaches to hyphae in an avid and prolonged manner, suggesting that Pf4-mediated inhibition of may occur at the biofilm surface. We show that Pf4 binds iron, thus denying a crucial resource. Consistent with this, the inhibition of metabolism by Pf4 could be overcome with supplemental ferric iron, with preformed biofilm more resistant to reversal. To our knowledge, this is the first report of a bacterium producing a phage that inhibits the growth of a fungus and the first description of a phage behaving as an iron chelator in a biological system.

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2016-09-01
2021-07-30
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