1887

Abstract

A total of 218 actinobacteria strains were isolated from wild perennial liquorice plants L. and BAT. Based on morphological characteristics, 45 and 32 strains from and , respectively, were selected for further analyses. According to 16S rRNA sequence analysis, most of the strains belonged to genus and a few strains represented the rare actinobacteria and . A total of 39 strains from and 27 strains from showed antimicrobial activity against at least one indicator organism. The range of the antimicrobial activity of the strains isolated from and was similar. A total of 34 strains from and 29 strains from carried at least one of the genes encoding polyketide synthases, non-ribosomal peptide synthetase and FADH-dependent halogenase. In the type II polyketide synthase KSα gene phylogenetic analysis, the strains were divided into two major clades: one included known spore pigment production-linked KSα sequences and other sequences were linked to the production of different types of aromatic polyketide antibiotics. Based on the antimicrobial range, the isolates that carried different KSα types were not separated from each other or from the isolates that did not carry KSα. The incongruent phylogenies of 16S rRNA and KSα genes indicated that the KSα genes were possibly horizontally transferred. In all, the liquorice plants were a rich source of biocontrol agents that may produce novel bioactive compounds.

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2016-07-01
2024-03-28
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