1887

Abstract

Two homologous proteins, UxuR and ExuR, were previously predicted to repress synthesis of enzymes required for hexuronic acid metabolism, but little is known about the relative roles of these proteins in gene regulation. We confirmed the previous report that UxuR is essential for rapid growth with -glucuronate as the primary source of carbon and energy. In contrast, an mutant grew more rapidly on -glucuronate than the parent. Transcription of is initiated at a σ–dependent promoter predicted . Purified ExuR bound to the regulatory region in the presence, but not in the absence, of -glucuronate. Apparently weaker UxuR binding in the presence of glucuronate was also detected, and its addition decreased ExuR binding by forming ExuR–UxuR heterodimers. Glucuronate induced transcription in the parental strain, but not in the mutant. No evidence was obtained for cAMP-dependent regulation of by the catabolite repressor protein (CRP). A previous study reported that the divergent and genes, essential for -galactonate metabolism, are repressed by UxuR. We showed that ExuR binds to the regulatory region, and that the binding is also glucuronate-dependent. As for the promoter, UxuR appeared to decrease ExuR binding. ExuR is required for glucuronate induction of and , and CRP is required for their transcription. The combined data established that UxuR and ExuR fulfil contrasting roles in regulating hexuronic acid metabolism and indicate that ExuR can function as a transcription activator, possibly by inactivating the repressor function of UxuR by heterodimer formation.

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2016-07-01
2020-01-22
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