1887

Abstract

A hospitalized 73-year-old woman was infected with a strain, 12 B-r, isolated from the place of injection of a blood catheter. Another strain, 12 B-k, recognized as genomospecies 5 or 6, was isolated from the patient's faeces, which was an example of a nosocomial infection rather than an auto-infection. Serological investigation using ELISA and Western blotting showed that strain 12 B-k from faeces belonged to the O2 serogroup. Strain 12 B-r from the wound displayed cross-reactions with several O serogroups due to common epitopes on the core or O-specific parts of the lipopolysaccharide. Studies of the isolated 12 B-r O-specific polysaccharide by NMR spectroscopy revealed its close structural similarity to that of O8. The only difference in 12 B-r was the presence of an additional GlcNAc-linked phosphoethanolamine residue, which creates a putative epitope responsible for the cross-reactivity with O16. The new O-antigen form could appear as a result of adaptation of the bacterium to a changing environment. On the basis of the data obtained, we suggest division of the O8 serogroup into two subgroups: O8a for strains of various species that have been previously classified into the O8 serogroup, and O8a,b for 12 B-r, where ‘a’ is a common epitope and ‘b’ is a phosphoethanolamine-associated epitope. These findings further confirm serological and structural heterogeneity of O antigens of strains isolated lately from patients in Poland.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.000274
2016-05-01
2020-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/162/5/789.html?itemId=/content/journal/micro/10.1099/mic.0.000274&mimeType=html&fmt=ahah

References

  1. Arbatsky N. P., Shashkov A. S., Widmalm G., Knirel Y. A., Zych K., Sidorczyk Z.. 1997; Structure of the O-specific polysaccharide of Proteus penneri strain 25 containing N-(L-alanyl) and multiple O-acetyl groups in a tetrasaccharide repeating unit. Carbohydr Res298:229–235 [CrossRef][PubMed]
    [Google Scholar]
  2. Arbatsky N. P., Drzewiecka D., Palusiak A., Shashkov A. S., Zabłotni A., Siwińska M., Knirel Y. A.. 2013; Structure of a Kdo-containing O polysaccharide representing Proteus O79, a newly described serogroup for some clinical Proteus genomospecies isolates from Poland. Carbohydr Res379:100–105 [CrossRef][PubMed]
    [Google Scholar]
  3. Bessa L. J., Fazii P., Di Giulio M., Cellini L.. 2015; Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. Int Wound J12:47–52 [CrossRef][PubMed]
    [Google Scholar]
  4. Drzewiecka D.. 2016; Significance and roles of Proteus spp. bacteria in natural environments. Microb Ecol [Epub ahead of print]. [CrossRef][PubMed]
    [Google Scholar]
  5. Drzewiecka D., Toukach P. V., Arbatsky N. P., Zych K., Shashkov A. S., Knirel Y. A., Sidorczyk Z.. 2002; Structure of the O-specific polysaccharide of Proteus penneri 103 containing ribitol and 2-aminoethanol phosphates. Carbohydr Res337:1535–1540 [CrossRef][PubMed]
    [Google Scholar]
  6. Drzewiecka D., Zych K., Sidorczyk Z.. 2004; Characterization and serological classification of a collection of Proteus penneri clinical strains. Arch Immunol Ther Exp (Warsz)52:121–128[PubMed]
    [Google Scholar]
  7. Drzewiecka D., Arbatsky N. P., Shashkov A. S., Stączek P., Knirel Y. A., Sidorczyk Z.. 2008; Structure and serological properties of the O-antigen of two clinical Proteus mirabilis strains classified into a new Proteus O77 serogroup. FEMS Immunol Med Microbiol54:185–194 [CrossRef][PubMed]
    [Google Scholar]
  8. Drzewiecka D., Arbatsky N. P., Stączek P., Shashkov A. S., Knirel Y. A., Sidorczyk Z.. 2010; Structural and serological studies of the O-polysaccharide of strains from a newly created Proteus O78 serogroup prevalent in Polish patients. FEMS Immunol Med Microbiol58:269–276 [CrossRef][PubMed]
    [Google Scholar]
  9. Gibbs K. A., Greenberg E. P.. 2011; Territoriality in Proteus: advertisement and aggression. Chem Rev111:188–194 [CrossRef][PubMed]
    [Google Scholar]
  10. Janda J. M., Abbott S. L., Khashe S., Probert W.. 2001; Biochemical identification and characterization of DNA groups within the Proteus vulgaris complex. J Clin Microbiol39:1231–1234 [CrossRef][PubMed]
    [Google Scholar]
  11. Knirel Y. A.. 2011; Structure of O-antigens. In Bacterial Lipopolysaccharides. Structure, Chemical Synthesis, Biogenesis and Interaction with Host Cells pp41–116 Edited by Knirel Y. A., Valvano M. A.. Vienna: Springer;
    [Google Scholar]
  12. Knirel Y. A., Zych K., Vinogradov E. V., Shashkov A. S., Sidorczyk Z.. 2000; Structure of a 2-aminoethyl phosphate-containing O-specific polysaccharide of Proteus penneri 8 from a new serogroup O67. Eur J Biochem267:815–820 [CrossRef][PubMed]
    [Google Scholar]
  13. Knirel Y. A., Perepelov A. V., Kondakova A. N., Senchenkova S. N., Sidorczyk Z., Różalski A., Kaca W.. 2011; Structure and serology of O-antigens as the basis for classification of Proteus strains. Innate Immun17:70–96 [CrossRef][PubMed]
    [Google Scholar]
  14. Larsson P.. 1984; Serology of Proteus mirabilis and Proteus vulgaris . Methods Microbiol14:187–214 [CrossRef]
    [Google Scholar]
  15. Manos J., Belas R.. 2006; The genera Proteus, Providencia, and Morganella . In The Prokaryotes, 3rd edn. pp245–269 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E.. New York: Springer; [CrossRef]
    [Google Scholar]
  16. O'Hara C. M., Brenner F. W., Miller J. M.. 2000a; Classification, identification, and clinical significance of Proteus, Providencia, and Morganella . Clin Microbiol Rev13:534–546 [CrossRef][PubMed]
    [Google Scholar]
  17. O'Hara C. M., Brenner F. W., Steigerwalt A. G., Hill B. C., Holmes B., Grimont P. A. D., Hawkey P. M., Penner J. L., Miller J. M., Brenner D. J.. 2000b; Classification of Proteus vulgaris biogroup 3 with recognition of Proteus hauseri sp. nov., nom. rev. and unnamed Proteus genomospecies 4, 5 and 6. Int J Syst Evol Microbiol50:1869–1875 [CrossRef][PubMed]
    [Google Scholar]
  18. Ovchinnikova O. G., Różalski A., Liu B., Knirel Y. A.. 2013; O-antigens of bacteria of the genus providencia: structure, serology, genetics, and biosynthesis. Biochemistry (Mosc)78:798–817[CrossRef]
    [Google Scholar]
  19. Perepelov A. V., Babicka D., Shashkov A. S., Arbatsky N. P., Senchenkova S. N., Różalski A., Knirel Y. A.. 1999; Structure and cross-reactivity of the O-antigen of Proteus vulgaris O8. Carbohydr Res318:186–192 [CrossRef][PubMed]
    [Google Scholar]
  20. Różalski A., Kwil I., Torzewska A., Baranowska M., Stączek P.. 2007; [Proteus bacilli: features and virulence factors]. Postepy Hig Med Dosw (Online)61:204–219 (in Polish)[PubMed]
    [Google Scholar]
  21. Różalski A., Torzewska A., Moryl M., Kwil I., Maszewska A., Ostrowska K., Drzewiecka D., Zabłotni A., Palusiak A., other authors. 2012; Proteus sp. – an opportunistic bacterial pathogen – classification, swarming growth, clinical significance and virulence factors. Folia Biol Oecol8:1–17 [CrossRef]
    [Google Scholar]
  22. Senior B. W.. 1997; Media and tests to simplify the recognition and identification of members of the Proteeae . J Med Microbiol46:39–44 [CrossRef][PubMed]
    [Google Scholar]
  23. Sidorczyk Z., Zych K., Toukach F. V., Arbatsky N. P., Zabłotni A., Shashkov A. S., Knirel Y. A.. 2002; Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3. Eur J Biochem269:1406–1412 [CrossRef][PubMed]
    [Google Scholar]
  24. Siwińska M., Levina E. A., Ovchinnikova O. G., Drzewiecka D., Shashkov A. S., Różalski A., Knirel Y. A.. 2015; Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80. Carbohydr Res407:131–136 [CrossRef][PubMed]
    [Google Scholar]
  25. Torzewska A., Grabowski S., Kondakova A. N., Toukach F. V., Senchenkova S. N., Shashkov A. S., Arbatsky N. P., Knirel Y. A., Rózalski A., Kaca W.. 2006; Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35. Arch Immunol Ther Exp (Warsz)54:277–282 [CrossRef][PubMed]
    [Google Scholar]
  26. Toukach F. V., Arbatsky N. P., Shashkov A. S., Knirel Y. A., Zych K., Sidorczyk Z.. 2001; Structure of the O-specific polysaccharide of Proteus mirabilis O16 containing ethanolamine phosphate and ribitol phosphate. Carbohydr Res331:213–218 [CrossRef][PubMed]
    [Google Scholar]
  27. Toukach F. V., Kondakova A. N., Arbatsky N. P., Senchenkova S. N., Shashkov A. S., Knirel Y. A., Zych K., Różalski A., Sidorczyk Z.. 2002; New structures of the O-specific polysaccharides of bacteria of the genus Proteus. 1. Phosphate-containing polysaccharides. Biochemistry (Mosc)67:265–276 [CrossRef][PubMed]
    [Google Scholar]
  28. Tsai C.-M., Frasch C. E.. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem119:115–119 [CrossRef][PubMed]
    [Google Scholar]
  29. Wang L., Wang Q., Reeves P. R.. 2010; The variation of O-antigens in Gram-negative bacteria. In Endotoxins: Structure, Function and Recognition pp123–152 Edited by Wang X., Quinn P. J.. Dordrecht: Springer; [CrossRef]
    [Google Scholar]
  30. Westphal O., Jann K.. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem5:83–91
    [Google Scholar]
  31. Zych K., Perepelov A. V., Siwińska M., Knirel Y. A., Sidorczyk Z.. 2005; Structures of the O-polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups. FEBS J272:5536–5543 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.000274
Loading
/content/journal/micro/10.1099/mic.0.000274
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error