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Volume 162, Issue 5

Immunochemical characterization of the O antigens of two strains, O8-related antigen of 12 B-r and O2-related antigen of genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland Free

Abstract

A hospitalized 73-year-old woman was infected with a strain, 12 B-r, isolated from the place of injection of a blood catheter. Another strain, 12 B-k, recognized as genomospecies 5 or 6, was isolated from the patient's faeces, which was an example of a nosocomial infection rather than an auto-infection. Serological investigation using ELISA and Western blotting showed that strain 12 B-k from faeces belonged to the O2 serogroup. Strain 12 B-r from the wound displayed cross-reactions with several O serogroups due to common epitopes on the core or O-specific parts of the lipopolysaccharide. Studies of the isolated 12 B-r O-specific polysaccharide by NMR spectroscopy revealed its close structural similarity to that of O8. The only difference in 12 B-r was the presence of an additional GlcNAc-linked phosphoethanolamine residue, which creates a putative epitope responsible for the cross-reactivity with O16. The new O-antigen form could appear as a result of adaptation of the bacterium to a changing environment. On the basis of the data obtained, we suggest division of the O8 serogroup into two subgroups: O8a for strains of various species that have been previously classified into the O8 serogroup, and O8a,b for 12 B-r, where ‘a’ is a common epitope and ‘b’ is a phosphoethanolamine-associated epitope. These findings further confirm serological and structural heterogeneity of O antigens of strains isolated lately from patients in Poland.

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© 2016 The Authors
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2016-05-01
2024-04-19
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Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland
Microbiology 162, 789 (2016); https://doi.org/10.1099/mic.0.000274
/content/journal/micro/10.1099/mic.0.000274
/content/journal/micro/10.1099/mic.0.000274
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