1887

Abstract

Ribosome dimers are a translationally inactive form of ribosomes found in and many other bacterial cells. In this study, we found that the 70S ribosomes of dimerized during the early stationary phase and these dimers remained in the cytoplasm until regrowth was initiated. Ribosome dimerization during the stationary phase required the gene, which encodes a homologue of the hibernation-promoting factor (Hpf). The expression of was induced at an early stationary phase and its expression was observed throughout the rest of the experimental period, including the entire 6 h of the stationary phase. Ribosome dimerization followed the induction of in WT cells, but the dimerization was impaired in cells harbouring a deletion in the gene. Although the absence of ribosome dimerization in these Hpf-deficient cells did not affect their viability in the stationary phase, their ability to regrow from the stationary phase decreased. Thus, following the transfer of stationary-phase cells to fresh LB medium, Δ mutant cells grew slower than WT cells. This observed lag in growth of Δ cells was probably due to a delay in restoring their translational activity. During regrowth, the abundance of ribosome dimers in WT cells decreased with a concomitant increase in the abundance of 70S ribosomes and growth rate. These results suggest that the ribosome dimers, by providing 70S ribosomes to the cells, play an important role in facilitating rapid and efficient regrowth of cells under nutrient-rich conditions.

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2016-03-01
2021-07-25
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