1887

Abstract

We analysed the ability of five different rhodococcal species to grow and produce triacylglycerols (TAGs) from glycerol, the main byproduct of biodiesel production. and grew fast on glycerol, whereas and exhibited a prolonged lag phase of several days before growing. only exhibited poor growth on glycerol. DSMZ 43060 and F7 produced 3.9–4.3 g cell biomass l and 28.4–44.6 % cellular dry weight (CDW) of TAGs after 6 days of incubation; whereas PD630 and RHA1 produced 2.5–3.8 g cell biomass l and 28.3–38.4 % CDW of TAGs after 17 days of growth on glycerol. Genomic analyses revealed two different sets of genes for glycerol uptake and degradation (here named clusters 1 and 2) amongst rhodococci. Those species that possessed cluster 1 () ( and ) exhibited fast growth and lipid accumulation, whereas those that possessed cluster 2 () (, and ) exhibited delayed growth and lipid accumulation during cultivation on glycerol. Three glycerol-negative strains were complemented for their ability to grow and produce TAGs by heterologous expression of from PD630. In addition, we significantly reduced the extension of the lag phase and improved glycerol assimilation and oil production of PD630 when expressing from . The results demonstrated that rhodococci are a flexible and amenable biological system for further biotechnological applications based on the reutilization of glycerol.

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2016-02-01
2019-10-14
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