1887

Abstract

Proferrorosamine A (proFRA) is an iron (Fe) chelator produced by the opportunistic plant pathogen P45. To identify genes involved in proFRA synthesis, transposon mutagenesis was performed. The identified 9.3 kb gene cluster, comprising seven genes, designated , encodes proteins that are involved in proFRA synthesis. Based on gene homologies, a biosynthetic pathway model for proFRA is proposed. To obtain a better understanding of the effect of proFRA on non-proFRA producing bacteria, P45 was co-cultured with CFBP1430, a fire-blight-causing plant pathogen. P45, but not corresponding proFRA-negative mutants, led to a pink coloration of CFBP1430 colonies on King's B agar, indicating accumulation of the proFRA–iron complex ferrorosamine, and growth inhibition . By saturating proFRA-containing extracts with Fe, the inhibitory effect was neutralized, suggesting that the iron-chelating capability of proFRA is responsible for the growth inhibition of CFBP1430.

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2016-02-01
2019-10-14
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