1887

Abstract

In 1982, (ss) was identified as the aetiological agent of Lyme disease. Since then an increasing number of (sl) species have been isolated in the United States. To date, many of these species remain understudied despite mounting evidence associating them with human illness. is a spirochaete closely related to that has been loosely associated with human illness. Using an experimental murine infection model, we compared the infectivity and humoral immune response with a North American isolate of and using culture, molecular and serological methods. The original cultures were unable to infect immunocompetent mice, but were confirmed to be infectious after adaptation in immunodeficient animals. infection resulted in spirochaete burdens similar to in skin, heart and bladder whereas significantly lower burdens were observed in the joint tissues. induced an antibody response similar to as measured by both immunoblotting and the C6 ELISA. Additionally, this isolate of was sequenced on the Ion Torrent PGM, which successfully identified many genes orthologous to mammalian virulence factors described in . Similarities seen between both infections in this well-characterized murine model contribute to our understanding of the potential pathogenic nature of . Infection dynamics of and especially the induced humoral response, are similar to , suggesting this species may contribute to the epidemiology of human borreliosis.

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2015-12-01
2021-06-18
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