In the CG43 genome, the divergently transcribed genes coding for PecS, the MarR-type transcription factor, and PecM, the drug metabolite transporter, are located between the type 1 and type 3 fimbrial gene clusters. The intergenic sequence between and contains three putative PecS binding sites and a CpxR box. Electrophoretic mobility shift assay revealed that the recombinant PecS and CpxR could specifically bind to the sequence, and the specific interaction of PecS and could be attenuated by urate. The expression of and was negatively regulated by CpxAR and PecS, and was inducible by exogenous urate in the absence of . Compared with CG43S3Δ, the derived mutants CG43S3ΔΔ and CG43S3ΔΔΔ exerted similar levels of sensitivity to HO or paraquat, but higher levels of mannose-sensitive yeast agglutination activity and FimA production. The promoter activity and transcript levels of in CG43S3Δ were also increased by deleting . However, no binding activity between PecS and the promoter could be observed. Nevertheless, PecS deletion could reduce the expression of the global regulator HNS and release the negative effect of HNS on FimA expression. In CG43S3Δ, the expression of FimA as well as PecS was inducible by urate, whilst urate-induced FimA expression was inhibited by the deletion of . Taken together, we propose that PecS indirectly and negatively regulates the expression of type 1 fimbriae, and the regulation is urate-inducible in the absence of CpxAR.


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