1887

Abstract

The putative zinc exporters ZntA (a P-type ATPase) and ZntB (2-TM-GxN family) in were characterized. The expression of the gene is inducible by CdCl, ZnCl and CoCl, of which CdCl is the most potent inducer, whereas is constitutively expressed. The metal-induced expression of is controlled by the MerR-like regulator ZntR. The and mutants were highly sensitive to CdCl and ZnCl, and CoCl sensitivity was demonstrated to a lesser extent. By contrast, the mutant showed similar levels of metal resistance to the WT strain. Even in the mutant background, did not play an apparent role in metal resistance under the conditions tested. The inactivation of increased the accumulation of intracellular cadmium and zinc, and conferred hyper-resistance to HO. Thus, the metal transporter ZntA and its regulator ZntR are important for controlling zinc homeostasis and cadmium and cobalt detoxification. The loss of either the or gene did not affect the virulence of in .

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2015-09-01
2019-10-18
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