1887

Abstract

We determined the distribution of microbial endosymbionts as possible agents of parthenogenesis in Oribatida. We screened mites from 20 species of 14 families suspected to be parthenogenetic from the absence or rarity of males. Our research included parthenogenesis-inducing bacteria spp., spp., spp., and additionally , and microsporidia that can also manipulate host reproduction. We detected the endosymbionts by PCR-based methods and transmission electron microscopy (TEM) observation of fixed and stained preparations of host cells. We detected only in one Oribatida species, , by identifying genes using PCR. TEM observations confirmed infection by the endosymbiont in and its lack in other Oribatida species. Sequence analysis of and genes showed that the strain from was different from strains characterized in some insects, crustaceans (Isopoda), mites (Tetranychidae), springtails (Hexapoda) and roundworms (Nematoda). The analysis strongly suggested that the sp. strain found in did not belong to supergroups A, B, C, D, E, F, H or M. We found that the sequences of from were clearly distantly related to sequences from the bacteria of the other supergroups. This observation makes a unique host in terms of the distinction of the strain. The role of these micro-organisms in remains unknown and is an issue to investigate.

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2015-08-01
2021-07-28
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