1887

Abstract

The aim of this study is to investigate aflatoxin gene expression during interaction. Aflatoxins are carcinogenic compounds produced mainly by and A previous study has shown that interaction can reduce aflatoxin content . Here, we first validated this same effect in the interaction with . Moreover, we showed that growth reduction and aflatoxin content were correlated in but not in . Secondly, we investigated the mechanisms of action by reverse-transcriptase quantitative PCR. As microbial interaction can lead to variations in expression of household genes, the most stable [, β (and for )] were chosen using geNorm software. To shed light on the mechanisms involved, we studied during the interaction the expression of five genes (, , , and ). Overall, the results of aflatoxin gene expression showed that repressed gene expression to a greater level in than in Expression of and was generally repressed in both species Expression of was repressed and was correlated with aflatoxin B1 content. The results suggest that expression could be a potential aflatoxin indicator in species interactions. Therefore, we demonstrate that can reduce aflatoxin production by both species and that this effect can be correlated with the repression of expression.

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2015-05-01
2019-10-23
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