1887

Abstract

Besides the canonical phosphoenolpyruvate-dependent phosphotransferase system (PTS) for carbohydrate transport, most possess the so-called nitrogen PTS (PTS) that transfers a phosphate group from phosphoenolpyruvate (PEP) over enzyme I (EI) and NPr to enzyme IIA (EIIA). The PTS lacks membrane-bound components and functions exclusively in a regulatory capacity. While EIIA has been implicated in a variety of cellular processes such as potassium homeostasis, phosphate starvation, nitrogen metabolism, carbon metabolism, regulation of ABC transporters and poly-β-hydroxybutyrate accumulation in many , the only identified role of NPr is the regulation of biosynthesis of the lipopolysaccharide (LPS) layer by direct interaction with LpxD in . In this study, we provide another phenotype related to NPr. Several lines of evidence demonstrate that strains with increased levels of dephosphorylated NPr are sensitive to envelope stresses, such as osmotic, ethanol and SDS stresses, and these phenotypes are independent of LpxD. The C-terminal region of NPr plays an important role in sensitivity to envelope stresses. Thus, our data suggest that the dephospho-form of NPr affects adaptation to envelope stresses through a C-terminus-dependent mechanism.

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2015-05-01
2024-03-29
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