Many strains of lactic acid bacteria have been used for the production of probiotics. Some metabolites produced by lactic acid bacteria impair the motilities of pathogenic bacteria. Because bacterial motility is strongly associated with virulence, the metabolic activities of lactic acid bacteria are effective for suppressing bacterial infections. Here we show that lactose fermentation by subsp. inhibits the motility of serovar Typhimurium. A single-cell tracking and rotation assay for a single flagellum showed that the swimming behaviour of was severely but transiently impaired through disruption of flagellar rotation on exposure to media cultivated with . Using a pH-sensitive fluorescent protein, we observed that the intracellular pH of was decreased because of some fermentation products of . We identified acetate as the lactose fermentation product of triggering the paralysis of flagella. The motilities of , and strains were also severely disrupted by lactose utilization by . These results highlight the potential use of for preventing infections by multiple bacterial species.


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