1887

Microbiology Society logo

Volume 161, Issue 4

A lactose fermentation product produced by subsp. , acetate, inhibits the motility of flagellated pathogenic bacteria Free

Abstract

Many strains of lactic acid bacteria have been used for the production of probiotics. Some metabolites produced by lactic acid bacteria impair the motilities of pathogenic bacteria. Because bacterial motility is strongly associated with virulence, the metabolic activities of lactic acid bacteria are effective for suppressing bacterial infections. Here we show that lactose fermentation by subsp. inhibits the motility of serovar Typhimurium. A single-cell tracking and rotation assay for a single flagellum showed that the swimming behaviour of was severely but transiently impaired through disruption of flagellar rotation on exposure to media cultivated with . Using a pH-sensitive fluorescent protein, we observed that the intracellular pH of was decreased because of some fermentation products of . We identified acetate as the lactose fermentation product of triggering the paralysis of flagella. The motilities of , and strains were also severely disrupted by lactose utilization by . These results highlight the potential use of for preventing infections by multiple bacterial species.

  • Received:
  • Accepted:
  • Published Online:
© 2015 The Authors
Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.000031
2015-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/161/4/701.html?itemId=/content/journal/micro/10.1099/mic.0.000031&mimeType=html&fmt=ahah

References

  1. Berg H. C.(2003). The rotary motor of bacterial flagella. Annu Rev Biochem 72, 19–54. [View Article][PubMed] [Google Scholar]
  2. Chevance F. F., Hughes K. T.(2008). Coordinating assembly of a bacterial macromolecular machine. Nat Rev Microbiol 6, 455–465. [View Article][PubMed] [Google Scholar]
  3. FAO/WHO (2001). Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria, Córdoba, Argentina, 1–4 October 2001. Rome: Food and Agriculture Organization of the United Nations, World Health Organization. Available at ftp://ftp.fao.org/es/esn/food/probio_report_en.pdf
  4. Fukuda S., Toh H., Taylor T. D., Ohno H., Hattori M.(2012). Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters. Gut Microbes 3, 449–454. [View Article][PubMed] [Google Scholar]
  5. Garvie E. I., Farrow J. A. E.(1982). Streptococcus lactis subsp. cremoris (Orla-Lensen) comb. nov. and Streptococcus lactis subsp. diacetilactis (Matuszewski et al.) nom. rev., comb. nov.Int J Syst Bacteriol 32, 453–455. [View Article] [Google Scholar]
  6. Hugenholtz J., Kleerebezem M., Starrenburg M., Delcour J., de Vos W., Hols P.(2000). Lactococcus lactis as a cell factory for high-level diacetyl production. Appl Environ Microbiol 66, 4112–4114. [View Article][PubMed] [Google Scholar]
  7. Johnson D. E., Ai H. W., Wong P., Young J. D., Campbell R. E., Casey J. R.(2009). Red fluorescent protein pH biosensor to detect concentrative nucleoside transport. J Biol Chem 284, 20499–20511. [View Article][PubMed] [Google Scholar]
  8. Josenhans C., Suerbaum S.(2002). The role of motility as a virulence factor in bacteria. Int J Med Microbiol 291, 605–614. [View Article][PubMed] [Google Scholar]
  9. Kihara M., Macnab R. M.(1981). Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria. J Bacteriol 145, 1209–1221.[PubMed] [Google Scholar]
  10. Lertsethtakarn P., Ottemann K. M., Hendrixson D. R.(2011). Motility and chemotaxis in Campylobacter and Helicobacter. Annu Rev Microbiol 65, 389–410. [View Article][PubMed] [Google Scholar]
  11. Liévin-Le Moal V., Amsellem R., Servin A. L.(2011). Impairment of swimming motility by antidiarrheic Lactobacillus acidophilus strain LB retards internalization of Salmonella enterica serovar Typhimurium within human enterocyte-like cells. Antimicrob Agents Chemother 55, 4810–4820. [View Article][PubMed] [Google Scholar]
  12. Liévin-Le Moal V., Fayol-Messaoudi D., Servin A. L.(2013). Compound(s) secreted by Lactobacillus casei strain Shirota YIT9029 irreversibly and reversibly impair the swimming motility of Helicobacter pylori and Salmonella enterica serovar Typhimurium, respectively. Microbiology 159, 1956–1971. [View Article][PubMed] [Google Scholar]
  13. Minamino T., Imae Y., Oosawa F., Kobayashi Y., Oosawa K.(2003). Effect of intracellular pH on rotational speed of bacterial flagellar motors. J Bacteriol 185, 1190–1194. [View Article][PubMed] [Google Scholar]
  14. Nakamura S., Kami-ike N., Yokota J. P., Kudo S., Minamino T., Namba K.(2009). Effect of intracellular pH on the torque-speed relationship of bacterial proton-driven flagellar motor. J Mol Biol 386, 332–338. [View Article][PubMed] [Google Scholar]
  15. Rasband, W. S. (1997–2014). ImageJ. Bethesda, MD: U. S. National Institutes of Health. http://imagej.nih.gov/ij/.
  16. Repaske D. R., Adler J.(1981). Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents. J Bacteriol 145, 1196–1208.[PubMed] [Google Scholar]
  17. Yamaguchi S., Fujita H., Sugata K., Taira T., Iino T.(1984). Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella. J Gen Microbiol 130, 255–265.[PubMed] [Google Scholar]
  18. Yoshioka K., Aizawa S.-I., Yamaguchi S.(1995). Flagellar filament structure and cell motility of Salmonella typhimurium mutants lacking part of the outer domain of flagellin. J Bacteriol 177, 1090–1093.[PubMed] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.000031
Loading
A lactose fermentation product produced by Lactococcus lactis subsp. lactis, acetate, inhibits the motility of flagellated pathogenic bacteria
Microbiology 161, 701 (2015); https://doi.org/10.1099/mic.0.000031
/content/journal/micro/10.1099/mic.0.000031
/content/journal/micro/10.1099/mic.0.000031
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error