RT Journal Article SR Electronic(1) A1 Sueiro-Olivares, Mónica A1 Fernandez-Molina, Jimena V. A1 Abad-Diaz-de-Cerio, Ana A1 Gorospe, Eva A1 Pascual, Elisabeth A1 Guruceaga, Xabier A1 Ramirez-Garcia, Andoni A1 Garaizar, Javier A1 Hernando, Fernando L. A1 Margareto, Javier A1 Rementeria, AitorYR 2015 T1 Aspergillus fumigatus transcriptome response to a higher temperature during the earliest steps of germination monitored using a new customized expression microarray JF Microbiology, VO 161 IS 3 SP 490 OP 502 DO https://doi.org/10.1099/mic.0.000021 PB Microbiology Society, SN 1465-2080, AB Aspergillus fumigatus is considered to be the most prevalent airborne pathogenic fungus and can cause invasive diseases in immunocompromised patients. It is known that its virulence is multifactorial, although the mechanisms of pathogenicity remain unclear. With the aim of improving our understanding of these mechanisms, we designed a new expression microarray covering the entire genome of A. fumigatus. In this first study, we analysed the transcriptomes of this fungus at the first steps of germination after being grown at 24 and 37 °C. The microarray data revealed that 1249 genes were differentially expressed during growth at these two temperatures. According to our results, A. fumigatus modified significantly the expression of genes related to metabolism to adapt to new conditions. The high percentages of genes that encoded hypothetical or unclassified proteins differentially expressed implied that many as yet unknown genes were involved in the establishment of A. fumigatus infection. Furthermore, amongst the genes implicated in virulence upregulated at 37 °C on the microarray, we found those that encoded proteins mainly related to allergens (Asp F1, Asp F2 and MnSOD), gliotoxin biosynthesis (GliP and GliZ), nitrogen (NiiA and NiaD) or iron (HapX, SreA, SidD and SidC) metabolism. However, gene expression in iron and nitrogen metabolism might be influenced not only by heat shock, but also by the availability of nutrients in the medium, as shown by the addition of fresh medium., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000021