1887

Abstract

SUMMARY: In growing cultures of flagellar ε--methyllysine (NML) can be isotopically labelled with either L-[Me-C]methionine or L-[U-C]lysine. This isotopic labelling of flagellar NML was unaffected by the addition of DL-NML to the incubation medium. The methionine analogues, D-methionine and DL-ethionine, competitively inhibited the incorporation of the methyl group from methionine into flagellar NML, but had no effect on the incorporation of L-[U-C]lysine. Although it is probable that methylation of lysine occurs after it has been incorporated into the flagellin molecule, it has not been possible by using antibiotics and purine and pyrimidine analogues to differentiate between flagellar synthesis and the formation of flagellar NML. Attempts to obtain a cell-free preparation capable of synthesizing NML have so far been unsuccessful.

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1966-01-01
2024-04-25
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References

  1. Allfrey V. G., Faulkner R., Mirsky A. E. 1964; Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc. natn. Acad. Sci. V.S.A 51:789
    [Google Scholar]
  2. Ambler R. P., Rees M. W. 1959; є-N-methyl-lysine in bacteria flagellar protein. Nature, Lond 184:56
    [Google Scholar]
  3. Blumenstein J., Williams G. R. 1963; Glycine methyltransferase. Can. J. Biochem. Physiol 41:201
    [Google Scholar]
  4. Fleissner E., Borek E. 1963; Studies on the enzymatic methylation of soluble RNA. I. Methylation of the s-RNA polymer. Biochemistry, N.Y 2:1093
    [Google Scholar]
  5. Gale E. F. 1963; Mechanisms of antibiotic action. Pharm. Rev 15:481
    [Google Scholar]
  6. Hurwitz J., Fürth J. S., Malamy M., Alexander M. 1962; The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by Actinomycin D and proflavin. Proc. natn. Acad. Sci. U.S.A 48:1222
    [Google Scholar]
  7. Iino T., Mitani M. 1962; Effect of phenol on the flagellation of Salmonella cell. Rep. natn. Inst. Genet., Misima 13:74
    [Google Scholar]
  8. Kerridge D. 1959; Synthesis of flagella by amino acid requiring mutants of Salmonella typhimurium. J. gen. Microbiol 21:168
    [Google Scholar]
  9. Kerridge D. 1962; The incorporation of “C labelled methionine into bacterial flagella. J. gen. Microbiol 31:xxiv
    [Google Scholar]
  10. Kerridge D. 1963; Flagellar synthesis in Salmonella typhimurium. The incorporation of isotopically labelled amino acids into flagellin. J. gen. Microbiol 33:63
    [Google Scholar]
  11. Kirk J. M. 1960; The mode of action of Actinomycin D. Biochim. biophys. Acta 42:167
    [Google Scholar]
  12. Kramer E., Koch F. E. 1931; Hemmung des Schwärmens der Proteuskolonien durch Chloralhydrat, verglichen mit der Hemmung durch Dextrose, 42°-Züchtung, Phenol, Natrium-chloride, Wismut, Esbach Reagenz, Malachitgrün, Galle und Kupfer-sulphat. Zentbl. Bakt. (Orig. Abt. 1) 120:452
    [Google Scholar]
  13. Leive L. 1965; Actinomycin sensitivity in Escherichia coli produced by EDTA. Biochem. biophys. Res. Com 18:13
    [Google Scholar]
  14. Mandel H. G., Latimer R. G., Rns M. 1965; The actions of thioguanine in Bacillus cereus. Biochem. Pharmacol 14:661
    [Google Scholar]
  15. Murray K. 1964; The occurrence of є-N-methyllysine in histones. Biochemistry, N.Y 3:10
    [Google Scholar]
  16. Stocker B. A. D., Campbell J. C. 1959; The effect of non-lethal deflagellation on bacterial motility and observations on flagellar regeneration. J. gen. Microbiol 20:670
    [Google Scholar]
  17. Stocker B. A. D., McDonough M. W., Ambler R. P. 1961; A gene determining presence or absence of є-N-methyllysine in Salmonella flagellar protein. Nature, Lond 189:556
    [Google Scholar]
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