@article{mbs:/content/journal/micro/10.1099/13500872-145-7-1613, author = "Sudoh, Masayuki and Tatsuno, Kenji and Ono, Naomi and Ohta, Atsunori and Chibana, Hiroji and Yamada-Okabe, Hisafumi and Arisawa, Mikio", title = "The Candida albicans CHS4 gene complements a Saccharomyces cerevisiae skt5/chs4 mutation and is involved in chitin biosynthesis", journal= "Microbiology", year = "1999", volume = "145", number = "7", pages = "1613-1622", doi = "https://doi.org/10.1099/13500872-145-7-1613", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-145-7-1613", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "CHS4", keywords = "Candida albicans", keywords = "chitin synthesis", abstract = "The Candida albicans CHS4 gene encoding chitin synthase 4 has been isolated using the Saccharomyces cerevisiae CHS4/SKT5 gene as a probe. The gene contains a 2061 bp open reading frame capable of encoding a protein of 687 amino acids (76053 Da). No intron was observed in the gene. Disruption of CHS4 in C. albicans yielded a Calcofluor-resistant phenotype, indicating that Chs4p contributes to chitin biosynthesis. Consistent with this, overexpression of Chs4p under the regulation of the ScGAL1 promoter enhanced chitin synthase 3 activity in S. cerevisiae 7- to 38-fold. In addition, chs3 and chs4 null mutants were significantly defective in Calcofluor white staining and their chitin content was 10% of that of the parental strain. Chs4p of C. albicans and S. cerevisiae showed 61 % identity in the C-terminal half of the proteins and that region of C. albicans Chs4p complemented the Chs4p function of a mutant of S. cerevisiae resistant to Calcofluor white. Therefore, it appears that Chs4p is involved in chitin synthase 3 activity by combining with Chs3p to interact synergistically in chitin biosynthesis.", }