1887

Abstract

The acetan biosynthetic pathway in is an ideal model system for engineering novel bacterial polysaccharides. To genetically manipulate this pathway, an strain (CKE5), more susceptible to gene-transfer methodologies, was developed. A new gene, involved in acetan biosynthesis was identified, sequenced and shown to have homology at the amino acid level with β-D-glucosyl transferases from a number of different organisms. Disruption of in strain CKE5 confirmed the function assigned above and was used to engineer a novel polysaccharide with a pentasaccharide repeat unit.

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/content/journal/micro/10.1099/13500872-145-6-1499
1999-06-01
2019-11-18
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-145-6-1499
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