Four genes, and of the DMSO respiratory gene cluster of have been identified and sequenced. encodes a pentahaem c-type cytochrome of the NirT class and the derived DorC protein sequence shows highest similarity to TorC from the trimethylamine--oxide (TMAO) respiratory system. Mutagenesis of resulted in the loss of a 46 kDa haem-staining polypeptide from membranes of encodes a protein with highest sequence similarity to TorD from the TMAO respiratory system. DMSO reductase polypeptide (DorA could not be detected in cell-free extracts of a mutant and it is suggested that DorD has a role in stabilizing the DorA apo-protein prior to insertion of the pterin molybdenum cofactor. encodes a protein with highest sequence similarity to NapD of Mutagenesis of reduced the activity of DMSO reductase and led to the accumulation of a large form of the enzyme that is presumed to represent a cytoplasmic precursor polypeptide. It is suggested that DorB has a role in the biogenesis of DMSO reductase prior to its secretion into the periplasm. is transcribed in the opposite direction to The derived amino acid sequence of DorR indicates that it is a response regulator and mutation of shows that it is essential for expression of the operon. Expression of a chromosomal fusion was also dependent on the transcriptional regulator Fnr. The intergenic region between and contains four putative binding sites for DorR but no binding site for Fnr was identified.


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