RT Journal Article SR Electronic(1) A1 Ferreira, Ana Margarida A1 Wait, Robin A1 Nobre, M. Fernanda A1 Costa, Milton S. daYR 1999 T1 Characterization of glycolipids from Meiothermus spp. JF Microbiology, VO 145 IS 5 SP 1191 OP 1199 DO https://doi.org/10.1099/13500872-145-5-1191 PB Microbiology Society, SN 1465-2080, AB Thin-layer chromatographic analysis of the polar lipids of Meiothermus strains revealed two glycolipid bands with similar chromatographic mobility to the major glycolipid of Thermus strains. In this study the glycolipids from the type strains of Meiothermus ruber, Meiothermus chliarophilus, Meiothermus silvanus and Meiothermus cerbereus were characterized using GC, GC/MS, fast atom bombardment MS and chemical methods. All strains contained dihexosyl-(N-acyl)hexosaminylglucosyl diacylglycerols, related in structure to the major glycolipid of Thermus strains but varying in their fatty acylation pattern. The detection of two glycolipid bands by TLC in Meiothermus spp. was attributable to the invariable presence of 2-hydroxyacyl groups N-linked to the hexosamine of the polar head group which cause the glycolipids to be more strongly retained on silica TLC plates than 3-hydroxy or non-hydroxylated N-acyl glycolipids of similar structure that are also present. M. silvanus contained, in addition to these glyceroglycolipids, several glycolipids which were linked to acylated branched octadecanediols rather than to glycerol. The presence of glycolipids containing 2-hydroxyacyl groups N-linked to hexosamine appears to be a stable phenotypic marker that distinguishes the genus Meiothermus from the genus Thermus. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-145-5-1191