@article{mbs:/content/journal/micro/10.1099/13500872-145-5-1173, author = "Arp, Daniel J.", title = "Butane metabolism by butane-grown ‘Pseudomonas butanovora’", journal= "Microbiology", year = "1999", volume = "145", number = "5", pages = "1173-1180", doi = "https://doi.org/10.1099/13500872-145-5-1173", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-145-5-1173", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "‘Pseudomonas butanovora’", keywords = "alkane metabolism", keywords = "butane metabolism", keywords = "alkane oxidation", abstract = "The pathway of butane metabolism by butane-grown ‘Pseudomonas butanovora' was determined to be butane → 1-butanol → butyraldehyde → butyrate. Butane was initially oxidized at the terminal carbon to produce 1-butanol. Up to 90% of the butane consumed was accounted for as 1-butanol when cells were incubated in the presence of 5 mM 1-propanol (to block subsequent metabolism of 1-butanol). No production of the subterminal oxidation product, 2-butanol, was detected, even in the presence of 5 mM 2-pentanol (an effective inhibitor of 2-butanol consumption). Ethane, propane and pentane, but not methane, were also oxidized. Butane-grown cells consumed 1-butanol and other terminal alcohols. Secondary alcohols, including 2-butanol, were oxidized to the corresponding ketones. Butyraldehyde was further oxidized to butyrate as demonstrated by blocking butyrate metabolism with 1 mM sodium valerate. Butyrate also accumulated from butane when cells were incubated with 1 mM sodium valerate. The pathway intermediates (butane, 1-butanol, butyraldehyde and butyrate) and 2-butanol stimulated O2 consumption by butane-grown cells. 1-Butanol, butyraldehyde and butyrate supported growth of ‘P. butanovora’, as did 2-butanol and lactate.", }