Summary: The authors have previously reported the nucleotide sequence of the 5′ and 3′ portions of the type 23F capsular polysaccharide biosynthesis locus () (from to and from to ). These regions of were very similar to the sequence reported for the capsule locus of type 19F. However, Southern hybridization analysis indicated that no other genes closely related to are present in the locus. In this study long-range PCR was used to amplify and clone the section of the type 23F capsule locus between and This region is 13 kb in size and contains 12 new ORFs, designated and Functions are proposed for all of the protein products, including functional homologues of Cps19fC-E, Cps19fI and Cps19fJ. A biosynthetic pathway for type 23F capsular polysaccharide is also proposed.


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