Summary: ATCC 17933 grown aerobically on ethanol produces a soluble cytochrome together with a quinoprotein ethanol dehydrogenase. A 3·2 kb genomic DNA fragment containing the gene encoding cytochrome was cloned and sequenced. Two other complete and two truncated ORFs were also identified. A truncated ORF encoding the quinoprotein ethanol dehydrogenase () was found upstream of the cytochrome gene () and in reverse orientation. An ORF encoding a NAD-dependent acetaldehyde dehydrogenase () was located downstream of the cytochrome gene and in the same orientation. Another ORF showed similarity to the gene and a truncated ORF similarity to the gene, both involved in the biosynthesis of the prosthetic group PQQ. The organization of these genes was found to be different from the well-studied methanol oxidation system in methylotrophic bacteria. The deduced amino acid sequence of cytochrome from showed some similarity to cytochrome of the alga and the haem domain of quinohaemoprotein alcohol dehydrogenases of acetic acid bacteria, but no similarity to the soluble cytochrome of the quinoprotein methanol oxidation system of methylotrophs could be detected. A mutant of with an interrupted cytochrome gene was unable to grow on ethanol, which proves that cytochrome is an essential component of the ethanol oxidation system in this organism.


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