Summary: serovar Typhimurium () is an enteric pathogen that causes significant morbidity in humans and other mammals. During their life cycle, salmonellae must survive frequent exposures to a variety of environmental stresses, e.g. carbon-source (C) starvation. The starvation-stress response (SSR) of encompasses the genetic and physiological realignments that occur when an essential nutrient becomes limiting for bacterial growth. The function of the SSR is to produce a cell capable of surviving long-term starvation. This paper reports that three C-starvation-inducible fusions from an C-starvation-inducible fusion library are all within a gene identified as which encodes an acyl-CoA dehydrogenase (ACDH) specific for medium-/long-chain fatty acids. This identification is supported by several findings: (a) significant homology at the amino acid sequence level with the ACDH enzymes from other bacteria and eukaryotes, (b) undetectable β-oxidation levels in insertion mutants, (c) inability of insertion mutants to grow on oleate or decanoate as a sole C-source, and (d) inducibility of fusions by the long-chain fatty acid oleate. In addition, the results indicate that the C-starvation-induction of is under negative control by the FadR global regulator and positive control by the cAMP:cAMP receptor protein complex and ppGpp. It is also shown that the locus is important for C-starvation-survival in Furthermore, the results demonstrate that is induced within cultured Madin-Darby canine kidney (MDCK) epithelial cells, suggesting that signals for its induction (C-starvation and/or long-chain fatty acids) may be present in the intracellular environment encountered by However, insertion mutations did not have an overt effect on mouse virulence.


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