Group A streptococci bind the serine protease plasmin with high affinity. Previously, a 41 kDa protein was identified as a candidate plasmin receptor protein (Plr) from group A streptococcal strain 64/14. The plr gene encoding Plr was cloned and the deduced amino acid sequence of Plr had significant similarity to glyceraldehyde-3-phosphate dehydrogenases (GAPDHs). In this study we have isolated cytoplasmic GAPDH of streptococcal strain 64/14. This enzyme was examined, on both structural and functional levels, for its relatedness to the Plr of strain 64/14 purified from mutanolysin extract and to recombinant Plr. We report here that no differences were detected between streptococcal Plr and cytoplasmic GAPDH on the basis of antibody reactivity, plasmin-binding activity, GAPDH activity, N-terminal amino acid sequence, peptide map analysis by V8 protease digestion and amino acid composition analysis. Furthermore, the plr gene appears to be present as a single copy in group A streptococci.
AlefounderP.R.,
PerhamR.N.1989; Identification, molecular cloning and sequence analysis of a gene cluster encoding the Class II fructose 1,6-biphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.
. Mol Microbiol 3:723–732
Ben NasarA.,
WistedtA.,
RingdahlU.,
SjobringU.1994; Streptokinase activates plasminogen bound to human group C and G streptococci through M-like proteins. Eur J Biochem 222:267–276
BranlantG.,
FleschG.,
BranlantC.1983; Molecular cloning of the glyceraldehyde-3-phosphate dehydrogenase genes of Bacillus stearothermophilus and Escherichia coli , and their expression in Escherichia coli.
. Gene 25:1–7
BroderC.C.,
LottenbergR.,
von MeringG.O.,
JohnstonK.H.,
BoyleM.D.P.1991; Isolation of a prokaryotic plasmin receptor: relationship to a plasminogen activator produced by the same micro-organism. J Biol Chem 266:4922–4928
BroesekerT.A.,
BoyleM.D.P.,
LottenbergR.1988; Characterization of the interaction of human plasmin with its specific receptor on a group A streptococcus. Microb Pathog 5:19–27
ClevelandD.W.,
FischerS.G.,
KirschnerM.W.,
LaemmliU.K.1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106
FernandesP.A.,
KeenJ.N.,
FindlayJ.B.C.,
Moradas-FerreiraP.1992; A protein homologous to glyceraldehyde-3-phosphate dehydrogenase is induced in the cell wall of a flocculent Kluyveromyces marxianus.
. Biochim Biophys Acta 1159:67–73
FuchsH.,
WallichR.,
SimonM.M.,
KramerM.D.1994; The outer surface protein A of the spirochete Borrelia burgdorferi is a plasmin(ogen) receptor. Proc Natl Acad Sci USA9112594–12598
GalanJ.E.,
TimoneyJ.F.,
CurtissR.III1988; Expression and localization of the Streptococcus equi M-protein in Escherichia coli and Salmonella typhimurium.
. In Proceedings of the Fifth International Conference of Equine Infectious Diseases pp. 34–40PowellD.G.
Edited by Lexington, KY: University Press of Kentucky;
Goudot-CrozelV.,
CaillolD.,
DjabaliM.,
DesseinA.L.1989; The major parasite surface antigen associated with human resistance to schistosomiasis is a 37 kDa glyceraldehyde-3p-dehydrogenase. J Exp Med 170:2065–2080
HollandJ.P.1983; Homologous nucleotide sequence at the 5ʹ termini of messenger RNAs synthesized from the yeast enolase and glyceraldehyde-3-phosphate dehydrogenase gene families. J Biol Chem 258:5291–5299
MichelsP.A.M.,
MarchandoM.,
KohlL.,
AllertS.,
WierengaR.K.,
OpperdoesF.R.1991; The cytosolic and glycosomal isoenzymes of glyceraldehyde-3-phosphate dehydrogenase in Trypanosoma brucei have a distant evolutionary relationship. Eur J Biochem 198:421–428
NelsonK.,
WhittamT.S.,
SelanderR.K.1991; Nucleotide polymorphism and evolution in the glyceraldehyde-3-phosphate dehydrogenase gene {gap A) in natural populations of Salmonella and Escherichia coli.
. Proc Natl Acad Sci USA886667–6671
PancholiV.,
FischettiV.A.1992; A major surface protein on group A streptococci is a glyceraldehyde-3-phosphate dehydrogenase with multiple binding activity. J Exp Med 176:415–426
PancholiV.,
FischettiV.A.1993; Glyceraldehyde-3-phosphate dehydrogenase on the surface of group A streptococci is also an ADP-ribosylating enzyme. Proc Natl Acad Sci USA908154–8158
UllbergM.,
KuuselaP.,
KristeansenB.-E.,
KronvallG.1992; Binding of plasminogen to Neisseria meningitidis and Neisseria gonorrhoeae and formulation of surface-associated plasmin. J Infect Dis 166:1329–1334
WangH.,
LottenbergR.,
BoyleM.D.P.1994; Analysis of plasmin(ogen) acquisition by clinical isolates of group A streptococci incubated in human plasma. J Infect Dis 169:143–149