1887

Abstract

A monoclonal antibody specific for -1,2-linked oligomannosides was used to study the association of these residues with mannan and phospholipomannan (PLM) in relation to growth conditions and in mannan mutant strains. Double immunofluorescence assays performed on cells grown under standard conditions indicated a highly heterogeneous cell surface expression of these epitopes in comparison with the homogeneous expression of α-linked oligomannosidic epitopes. Growth in the presence of tunicamycin, which inhibits mannan -glycosylation, resulted in an absence of -1,2-oligomannosidic epitopes on the cell surface, although PLM synthesis still occurred as shown by autoradiography. Similarly, growth in acidic conditions, which inhibits the incorporation of -1,2-oligomannosides in mannan, resulted in an absence of -1,2-oligomannosidic epitopes at the cell surface, although they still associated with PLM as shown by Western blotting. Western blots of mutant strains with reduced amounts or an absence of phosphorus and acid-labile -1,2-oligomannosides in their mannan confirmed that the association of -1,2-linked oligomannosides with mannan and with PLM involves different mannosylation processes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2263
1996-08-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2263.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2263&mimeType=html&fmt=ahah

References

  1. Brawner D., Cutler J., Beatty W. 1990; Caveats in the investigation of form-specific molecules of Candida albicans. . Infect Immun 58:378–383
    [Google Scholar]
  2. Cantelli C., Trinel P.A., Bernigaud A., Jouault T., Polonelli L., Poulain D. 1995; Mapping of β-1,2-linked oligomannosidic epitopes among glycoconjugates of Candida species. Microbiology 141:2693–2697
    [Google Scholar]
  3. Cassone A., Torosantucci A., Boccanera M., Pellegrini G., Palma C., Malavasi F. 1988; Production and characterization of a monoclonal antibody to a cell surface glucomannoprotein constituent of Candida albicans and other pathogenic Candida species. J Med Microbiol 27:233–238
    [Google Scholar]
  4. Chaffin W., Collins B., Marx J., Cole T., Morrow K. 1993; Characterization of mutant strains of Candida albicans deficient in expression of a surface determinant. Infect Immun 61:3449–3458
    [Google Scholar]
  5. Elorza M.V., Marcilla A., Sentandreu R. 1988; Wall manno- proteins of the yeast and mycelial cells of Candida albicans: nature of the glycosidic bonds and polvdtspersity of their mannan moieties. J Gen Microbiol 134:2393–2403
    [Google Scholar]
  6. Fruit J., Cailliez J., Odds F., Poulain D. 1990; Expression of an epitope by surface glycoproteins of Candida albicans. Variability among species, strains and veast cells of the genus Candida. . J Med Vet Mycol 28:241–252
    [Google Scholar]
  7. Fukazawa Y. 1989; Antigenic structure of Candida albicans-. immunochemical basis of the serologic specificity of the mannans in yeasts. In Immunology of Fungal Diseases pp. 37–62 Kurstak E. Edited by New York: Marcel Dekker;
    [Google Scholar]
  8. Hernando F., Cailliez J.C., Trinel P.A., Faille C., Mackenzie D.W.R., Poulain D. 1993; Qualitative and quantitative differences in recognition patterns of Candida albicans protein and polysaccharide antigens by human sera. J Med Vet Mycol 31:227–237
    [Google Scholar]
  9. Jouault T., Bernigaud A., Lepage G., Trinel P.A., Poulain D. 1994; The Candida albicans phospholipomannan induces in vitro production of tumor necrosis factor-α from human and murine macrophages. Immunology 83:268–273
    [Google Scholar]
  10. Jouault T., Lepage G., Bernigaud A., Trinel P.A., Fradin C., Wieruszeski M., Strecker G., Poulain D. 1995; β-l,2 Linked oligomannosides from Candida albicans act as signals for tumor necrosis factor alpha production. Infect Immun 63:2378–2381
    [Google Scholar]
  11. Kobayashi H., Shibata N., Nakada M., Chaki S., Mizugami K., Ohkubo Y., Suzuki S. 1990; Structural study of cell wall phosphomannan of Candida albicans NIH B-792 (serotype B) strain, with special reference to 1H and 13C NMR analyses of acid-labile oligomannosyl residues. Arch Biochem Biophys 278:195–204
    [Google Scholar]
  12. Kobayashi H., Kojimara T., Takahashi K., Takikawa M., Takahashi S., Shibata N., Okawa Y., Suzuki S. 1991a; Structural determination of D-mannans of pathogenic yeasts Candida stellatoidea type I strains: TIMM 0310 and ATCC 11006 compared to IFO 1397. Carbohydr Res 214:131–145
    [Google Scholar]
  13. Kobayashi H., Giummelly P., Takayashi S., Ishida M., Sato J., Takaku M., Nishidate Y., Shibata N., Okawa Y., Suzuki S. 1991b; Candida albicans serotype A strains grown in yeast extract- added sabouraud liquid medium at pH 2·0, elaborating mannans without β-l,2 linkage and phosphate group. Biochem Biophys Res Commun 175:1003–1009
    [Google Scholar]
  14. Kobayashi H., Shibata N., Suzuki S. 1992a; Evidence for oligomannosyl residues containing both β-l,2 and ɑ-1,2 linkages as a serotype A-specific epitope(s) in mannans of Candida albicans. . Infect Immun 60:2106–2109
    [Google Scholar]
  15. Kobayashi H., Mitobe H., Takahashi K., Yamamoto T., Shibata N., Suzuki S. 1992b; Structural study of a cell wall mannan- protein complex of the pathogenic yeast Candida glabrata IFO 0622 strain. Arch Biochem Biophys 294:662–669
    [Google Scholar]
  16. Kobayashi H., Takaku M., Nishidate Y., Takahashi S., Takakawa M., Shibata N., Suzuki S. 1992c; Structure of the d- mannan of the pathogenic yeast, Candida stellatoidea ATCC 20408 (Type I) strain, in comparison with that of C. stellatoidea ATCC 36232 (Type II) strain. Carbohydr Res 231:105–116
    [Google Scholar]
  17. Kobayashi H., Matsuda K., Ikeda T., Suzuki M., Takayashi S., Suzuki A., Shibata N., Suzuki S. 1994a; Structures of the cell wall mannans of Candida tropicalis IFO 0199 and IFO 1647 yeast strains. Infect Immun 62:615–622
    [Google Scholar]
  18. Kobayashi H., Takayashi S., Shibata N., Miyauchi M., Ishida M., Sato J., Maeda K., Suzuki S. 1994b; Structural modification of cell wall mannans of Candida albicans serotype A strains grown in yeast extract-sabouraud liquid medium under acidic conditions. Infect Immun 62:968–973
    [Google Scholar]
  19. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680–685
    [Google Scholar]
  20. Lehle L., Tanner W. 1976; The specific site of tunicamycin inhibition in the formation of dolichol-bound N-acetylglucosamine derivates. FEBS Eett 71:167–170
    [Google Scholar]
  21. Li R.-K., Cutler J. 1991; A cell surface/plasma membrane antigen of Candida albicans. . J Gen Microbiol 137:455–464
    [Google Scholar]
  22. Li R.-K., Cutler J.E. 1993; Chemical definition of an epitope/ adhesin molecule on Candida albicans. . J Biol Chem 268:18293–18299
    [Google Scholar]
  23. Lindberg B., Leontein K., Lindquist U., Svenson S., Wrangsell G., Deel A., Rogers M. 1988; Structural studies of the O-antigenic polysaccharide of Salmonella thompson , serogroup C1(6,7). Carbohydr Res 174:313–322
    [Google Scholar]
  24. Molinari A., Gomez M., Crateri P., Torosantucci A., Cassone A., Aranda G. 1993; Differential cell surface expression of mannoprotein epitopes in yeast and mycelial forms of Candida albicans. . Eur J Cell Biol 60:146–153
    [Google Scholar]
  25. Oxley D., Wilkinson S. 1991; Structure of a mannan isolated from the lipopolysaccharide of the reference strain (S3255) from a new serogroup of Serratia marcescens. . Carbohydr Res 212:213–217
    [Google Scholar]
  26. Poulain D., Faille C., Delaunoy C., Jacquinot P., Trinel P., Poulain D. 1993; Probable presence of β(1–2)-linked oligomannosides that act as human immunoglobulin G3 epitopes and are distributed over Candida albicans 14- to 18-kilodalton antigen. Infect Immun 61:1164–1166
    [Google Scholar]
  27. Shibata N., Ichikawa T., Tojo M., Takahashi M., Ito N., Okubo Y., Suzuki S. 1985; Immunochemical study on the mannan of Candida albicans NIH A207, NIH B792, and J1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide. Arch Biochem Biophys 243:338–348
    [Google Scholar]
  28. Shibata N., Fukasawa S., Kobayashi H., Tojo M., Yonezu T., Ambo A., Ohkubo Y., Suzuki S. 1989; Structural analysis of phospho-d-mannan-protein complexes isolated from yeast and mold form cells of Candida albicans NIH A-207 serotype A strain. Carbohydr Res 187:239–253
    [Google Scholar]
  29. Shibata N., Arai M., Haga E., Kikuchi T., Najima M., Satoh T., Kobayashi H., Suzuki S. 1992; Structural identification of an epitope of antigenic factor 5 in mannans of Candida albicans NIH B- 792 (serotype B) and J-1012 (serotype A) as β-1,2-linked oligomannosyl residues. Infect Immun 60:4100–4110
    [Google Scholar]
  30. Shibata N., Kojima C., Satoh Y., Satoh R., Suzuki A., Kobayashi H., Suzuki S. 1993; Structural study of a cell-wall mannan of Saccharomyces kluyveri IFO 1865 strain. Presence of a branched side chain and β, 2-linkage. Eur J Biochem 217:1 –12
    [Google Scholar]
  31. Shimokawa O., Nakayama H. 1984; Isolation of a Candida albicans mutant with reduced content of cell wall mannan and deficient mannan phosphorylation. J Med Vet Mycol 22:315–321
    [Google Scholar]
  32. Suzuki M., Fukazawa Y. 1982; Immunochemical characterization of Candida albicans cell wall antigens: specific determinant of Candida albicans serotype A mannan. Microbiol Immunol 26:387–402
    [Google Scholar]
  33. Suzuki M., Takata Y., Oshie A., Tezuka A., Shibata N., Kobayashi H., Okawa Y., Suzuki S. 1995; Detection of β-l,2- mannosyltransferase in Candida albicans cells. FEBS Eett 373:275–279
    [Google Scholar]
  34. Tojo M., Shibata N., Ban Y., Suzuki S. 1990; Structure of d-mannan of Candida stellatoidea IFO 1397 strain. Comparison with that of the phospho-d-mannan of Candida albicans NIH B-792 strain. Carbohydr Res 199:215–226
    [Google Scholar]
  35. Trinel P.A., Faille C., Jacquinot P.M., Cailliez J.C., Poulain D. 1992; Mapping of Candida albicans oligomannosidic epitopes by using monoclonal antibodies. Infect Immun 60:3845–3851
    [Google Scholar]
  36. Trinel P.A., Borg Von Zepelin M., Lepage G., Jouault T., Mackenzie D.W.R., Poulain D. 1993; Isolation and preliminary characterization of the 14- to 18-kDa Candida albicans antigen as a phospholipomannan containing β-1,2 linked oligo-mannosides. Infect Immun 61:4398–4405
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2263
Loading
/content/journal/micro/10.1099/13500872-142-8-2263
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error