1887

Abstract

The topological distribution of two epitopes in the cell wall of , the kinetics of their incorporation into the regenerating protoplast wall, and the effect of different antibiotics upon their incorporation and localization have been studied. To do so, two monoclonal antibodies that react against an -glycosylated mannoprotein (1B12) and against a 1,6--glucan epitope (JRR1) were used. The results show that the JRR1 epitope is localized in an internal layer of the cell wall, in contrast to the 1B12 epitope, which is superficial, and that the incorporation of the JRR1 epitope into walls of regenerating protoplasts precedes that of the 1B12 epitope. The JRR1 epitope is normally found in the culture medium of control cells, but not in that of papulacandin-B-treated cells, and tunicamycin interferes with the incorporation of the 1B12 epitope into the cell walls. Finally, the results support the hypothesis that mannoproteins are not 1,6--glycosylated before their secretion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2255
1996-08-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2255.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2255&mimeType=html&fmt=ahah

References

  1. Bodey G.P. 1984; Candidiasis: a growing concern. Am J Med 77:1–48
    [Google Scholar]
  2. Calderone R.A., Braun P.C. 1991; Adherence and receptor relationships of Candida albicans . Microbiol Rev 55:1–20
    [Google Scholar]
  3. Chattaway F.W., Holmes M.R., Barlow A.J.E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans . J Gen Microbiol 51:367–376
    [Google Scholar]
  4. Debono M., Gordee R.S. 1994; Antibiotics that inhibit fungal cell wall development. Annu Rev Microbiol 48:471–497
    [Google Scholar]
  5. Dubois M., Gilles K.A., Hamilton J.K., Rebes P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
    [Google Scholar]
  6. Elorza M.V., Marcilla A., Sentandreu R. 1988; Wall mannoproteins of the yeast and mycelial cells of Candida albicans: nature of glycosidic bonds and the polydispersity of their mannan moieties. J Gen Microbiol 134:2393–2403
    [Google Scholar]
  7. Elorza M.V., Mormeneo S., García de la Cruz F., Gimeno C., Sentandreu R. 1989; Evidence for the formation of covalent bonds between macromolecules in the domain of the walls of Candida albicans mycelial cells. Biochem Biophys Res Commun 162:1118–1121
    [Google Scholar]
  8. Elorza M.V., Marcilla A., Sanjuán R., Mormeneo S., Sentandreu R. 1994; Incorporation of specific wall proteins during yeast and mycelial protoplast regeneration in Candida albicans . Arch Microbiol 161:145–151
    [Google Scholar]
  9. Font de Mora J., Herrero E., Sentandreu R. 1993; A kinetic study on the regeneration of Candida albicans protoplasts in the presence of cell wall synthesis inhibitors. FEMS Microbiol Lett 111:43–48
    [Google Scholar]
  10. Horisberger M., Vonlanthen M. 1977; Location of mannan and chitin on thin sections of budding yeasts with gold markers. Arch Microbiol 115:1–7
    [Google Scholar]
  11. Kapteyn J.C., Montijn R.C., Dijkgraaf G.J.P., Klis F.M. 1994; Identification of β-l,6-glucosylated cell wall proteins in yeast and hyphal forms of Candida albicans . Eur J Cell Biol 65:402–407
    [Google Scholar]
  12. Kapteyn J.C., Montijn R.C., Dijkgraaf G.J.P., Van den Ende H., Klis F.M. 1995a; Covalent association of β-l,3-glucan with β-1,6-glucosylated mannoproteins in cell walls of Candida albicans . J Bacteriol 177:3788–3792
    [Google Scholar]
  13. Kapteyn J.C., Dijkgraaf G.J.P., Montijn R.C., Klis F.M. 1995b; Glucosylation of cell wall proteins in regenerating spheroplasts of Candida albicans . FEMS Microbiol Lett 128:271–277
    [Google Scholar]
  14. Koch Y., Rademacher K.H. 1980; Chemical and enzymatic changes in the cell wall of Candida albicans and Saccharomyces cerevisiae by scanning electron microscopy. Can J Microbiol 26:965–970
    [Google Scholar]
  15. Lee K., Buckley H.R., Campbell C.C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans . Sabouraudia 13:148–153
    [Google Scholar]
  16. Marcilla A., Elorza M.V., Mormeneo S., Rico H., Sentandreu R. 1991; Candida albicans mycelial wall structure: supramolecular complexes released by Zymolyase, chitinase and β-mercaptoethanol. Arch Microbiol 155:312–319
    [Google Scholar]
  17. Marcilla A., Mormeneo S., Elorza M.V., Manclús J.J., Sentandreu R. 1993; Wall formation by Candida albicans yeast cells: synthesis, secretion and incorporation of two types of mannoproteins. J Gen Microbiol 139:2985–2993
    [Google Scholar]
  18. Meaden P., Hill K., Wagner J., Slipetz D., Sommer S.S., Bussey H. 1990; The yeast KRE5 gene encodes a probable endoplasmic reticular protein required for β-l,6-D-glucan synthesis and normal cell growth. Mol Cell Biol 10:3013–3019
    [Google Scholar]
  19. Mormeneo S., Marcilla A., Iranzo M., Sentandreu R. 1994; Structural mannoproteins released by β-elimination from Candida albicans cell walls. FEMS Lett 123:131–136
    [Google Scholar]
  20. Murgui A., Elorza M.V., Sentandreu R. 1986; Tunicamycin and papulacandin B inhibit incorporation of specific mannoproteins into the wall of Candida albicans regenerating protoplasts. Biochim Biophys Acta 841:215–222
    [Google Scholar]
  21. Notario V. 1982; β -Glucanases from Candida albicans: purification, characterization and the nature of their attachment to cell wall components. J Gen Microbiol 128:747–759
    [Google Scholar]
  22. Odds F.C. 1988 Candida and Candidosis. London:: Ballière Tindall. London.;
    [Google Scholar]
  23. Ponton J., Jones J.M. 1986; Analysis of cell wall extracts of Candida albicans by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot techniques. Infect Immun 53:565–572
    [Google Scholar]
  24. Roemer T., Delaney S., Bussey H. 1993; SKN1 and KRE6 define a pair of functional homologues encoding putative membrane proteins involved in β -glucan synthesis. Mol Cell Biol 13:4039–4048
    [Google Scholar]
  25. Sanjuán R., Zueco J., Stock R., Font de Mora J., Sentandreu R. 1995; Identification of glucan-mannoprotein complexes in the cell wall of Candida albicans using a monoclonal antibody that reacts with a (l,6)- β-glucan epitope. Microbiology 141:1545–1551
    [Google Scholar]
  26. Sentandreu R., Mormeneo S., Ruiz-Herrera J. 1994; Bio-genesis of the fungal cell wall. In The Mycota. I. Growth, Differentiation and Sexuality pp. 111–124 Wessels J.G.H., Meinhardt F. Edited by Berlin, Heidelberg:: Springer-Verlag.;
    [Google Scholar]
  27. Shepherd M.G., Gopal P.K. 1993 Dimorphic Fungi in Biology and Medicine pp. 153–168 Vanden Bossche H., Odds F.C., Kerridge D. Edited by New York & London:: Plenum Press.;
    [Google Scholar]
  28. Sullivan P.A., Chiew Y.Y., Molloy C., Templeton M.D., Shepherd M.G. 1983; An analysis of the metabolism and cell wall composition of Candida albicans during germ-tube formation. Can J Microbiol 29:1514–1525
    [Google Scholar]
  29. Traxler P., Fritz H., Fuhrer H., Richter W.J. 1980; Papula candins, a new family of antibiotics with antifungal activity. Structures of papulacandins A, B, C and D. J Antibiot 33:967–978
    [Google Scholar]
  30. Voller A., Bidwell D. 1986; Enzyme-linked immunoadsorbent assay. In Manual of Clinical Laboratory Immunology pp. 359–371 Rose M.R., Friedman H., Faehey J.L. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  31. Zlotnik H., Fernandez M.P., Bowers B., Cabib E. 1984; S. cerevisiae mannoproteins form an external cell wall layer that determines wall porosity. J Biomol NMR 3:1018–1026
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2255
Loading
/content/journal/micro/10.1099/13500872-142-8-2255
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error