1887

Abstract

is the only culturable vibrio associated with the chitinaceous carapace of the copepod (Fisher 1860) living in Ligurian coastal rock pools (Tyrrhenian Sea). The characteristics of the interaction between chitin particles and were studied by analysing strains isolated both from the copepod surface and from rock-pool water. The highest degree of attachment to chitin was observed at 20°, in the presence of 3% NaCI. Bacterial treatment with -acetylglucosamine and pronase E caused a reduction in attachment of 52–62% and 77–94%, respectively. Chitin pretreatment with either wheat germ agglutinin or membrane proteins (MPs) from caused a reduction in attachment, of 50–57% and 53–70%, respectively. No inhibition was observed when bacteria were pretreated with -glucose, -fucose or -fructose, or when chitin was pretreated with concanavalin A and DH5α MPs. MPs able to bind chitin were isolated and analysed by SDS-PAGE. Four chitin-binding proteins were visualized in all tested strains (53, 35, 20 and 14 kDa); these peptides may efficiently mediate attachment to chitin-containing substrates.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2181
1996-08-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2181.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2181&mimeType=html&fmt=ahah

References

  1. Alderman D.J. 1986; Diseases control in crustacean aquaculture. In Efficiency in Aquaculture Production: Disease Control . Proceedings of the 3rd International Conference of Aquafarming pp. 107–112 Grimaldi E., Rosenthal H. Edited by Milano: Seme S.p.A;
    [Google Scholar]
  2. Belas M. R., Caldwell R.R. 1982; Adsorption kinetics of laterally and polarly flagellated Vibrio . J Bacteriol 151:1568–1580
    [Google Scholar]
  3. Baumann P., Schubert R.H.W. 1984; Family II. Vibrionaceae . In Berge’s Manual of Systematic Bacteriology 1: pp. 516–550 Krieg N. R., Holt J. G. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Carli A., Pane L., Casareto L., Bertone S., Pruzzo C. 1993; Occurrence of Vibrio alginolyticus in Ligurian coast rock pools (Tyrrhenian Sea, Italy) and its association with the copepod Tigriopusfulvus (Fisher 1860). Appl Environ Microbiol 59:1960–1962
    [Google Scholar]
  5. Carli A., Fraschetti S., Magni P., Mariottini G.L, Pruzzo C., Romairone V. 1994; A study of planktonic populations in relation to the eutrophication state of the water in a ligurian sea zone. In Mediterranean Action Plan Technical Reports Series78 pp. 37–52 Athens: UNEP/FAO;
    [Google Scholar]
  6. Colwell R.R. 1981; Microbial ecology and biofouling. In Biotechnology in the Marine Sciences pp. 221–231 Colwell R. R., Pariser E. R., Siskey A. J. Edited by New York: Wiley Intersciences;
    [Google Scholar]
  7. El-Gamal A.A., Alderman D.J., Rodgers C.J., Polglase J.L., Macintosh D. 1986; A scanning electron microscope study of oxolinic acid treatment of burn spot lesion of Macrobrachium rasenbergii . Aquaculture 52:152–171
    [Google Scholar]
  8. Farmer J.J. III Hickman-Brenner F.W., Kelly M.T. 1991; Vibrio. In Manual of Clinical Microbiology, 4th ed.. pp. 281–301 Lennette E. H., Balows A., Hausler W. J., Shadomy H. J. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Gooday G.W., Prasser J.I., Hillman K., Cross M.G. 1991; Mineralization of chitin in an estuarine sediment: the importance of the chitosan pathway. Biochem Syst Ecol 19:395–400
    [Google Scholar]
  10. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–579
    [Google Scholar]
  11. Hirche H.-J., Mumm N. 1992; Distribution of dominant copepods in the Nansen Basin, Arctic Ocean, in summer. Deep-Sea Res 39:S485–S505
    [Google Scholar]
  12. Hopkins T.L. 1985; Food web of an Antarctic midwater ecosystem. Mar Biol 89:197–212
    [Google Scholar]
  13. Huq A., West P.A., Small E.B., Huq M.I., Colwell R.R. 1983; Ecological relationships between Vibrio cholerae and planktonic crustacean copepods. Appl Environ Microbiol 45:275–283
    [Google Scholar]
  14. Huq A., West P.A., Small E.B., Huq I., colwell R.R. 1984; Influence of water temperature, salinity, and pH on survival and growth of toxigenic Vibrio cholerae serovar Ol associated with live copepods in laboratory microcosmos. Appl Environ Microbiol 48:420–424
    [Google Scholar]
  15. Jannatipur M., Soto-Gil R.W., Childers L.C., Zynskind J.W. 1987; Translocation of Vibrio harveyi N,N ’-diacetylchitobiase to the outer membrane of Escherichia coli . J Bacteriol 169:3785–3791
    [Google Scholar]
  16. Jeuniaux C. 1966; Chitinases. Methods Enzymol 8:644–650
    [Google Scholar]
  17. Koneko T., Colwell R.R. 1975; Adsorption of Vibrio para- haemolyticus onto chitin and copepods. Appl Microbiol 29:269–274
    [Google Scholar]
  18. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  19. Miyamoto S., Yamamoto H., Seki H. 1991; Chitin dynamics in the freshwater environment. Biochem Syst Ecol 19:371–377
    [Google Scholar]
  20. Montgomery M.T., Kirchman D.L. 1993; Role of chitinbinding proteins in the specific attachment of the marine bacterium Vibrio harveyi to chitin. Appl Environ Microbiol 59:373–379
    [Google Scholar]
  21. Montgomery M.T., Kirchman D.L. 1994; Induction of chitin¬binding proteins during the specific attachment of the marine bacterium Vibrio harveyi to chitin. Appl Environ Microbiol 60:4284–4288
    [Google Scholar]
  22. Nalin D.R., Daya V., Reid A., Levine M.M., Wu H.C. 1979; Adsorption and growth of Vibrio cholerae on chitin. Infect Immun 25:768–770
    [Google Scholar]
  23. Roszak D.B., Colwell R.R. 1987; Survival strategies of bacteria in the natural environment. Microbiol Rev 51:365–379
    [Google Scholar]
  24. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd ed.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Singh S.N., Srivastava R., Sinha V.B, Srivastava S. 1994; A 53 kDa protein of Vibrio cholerae classical strain 00395 involved in intestinal colonization. Microb Pathog 17:69–78
    [Google Scholar]
  26. Smith D.C., Simson M., Alldredge A.L, Azam F. 1992; Intense hydrolytic enzyme activity on marine aggregates and implications for rapid particles dissolution. Nature 359:139–142
    [Google Scholar]
  27. Tamplin M.L., Gauzens A.L., Huq A., Sack D.A., Colwell R.R. 1990; Attachment of Vibrio cholerae serogroup Ol to zooplankton and phytoplankton of Bangladesh waters. Appl Environ Microbiol 56:1977–1980
    [Google Scholar]
  28. Yu C., Lee A.M., Roseman S. 1987; The sugar-specific adhesion/deadhesion apparatus of the marine bacterium Vibrio furnissii is a sensorium that continuously monitors nutrient levels in the environment. Biochem Biophys Res Cornrnm 149:86–92
    [Google Scholar]
  29. Yu C., Lee A.M., Bassler B.L., Roseman S. 1991; Chitin utilization by marine bacteria. A physiological function for bacterial adhesion to immobilized carbohydrates. J Biol Chem 25:24260–24267
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2181
Loading
/content/journal/micro/10.1099/13500872-142-8-2181
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error