1887

Abstract

A low-fibronectin-binding mutant of strain 879R4SSp generated by transposon Tn mutagenesis is attenuated in a rat endocarditis model (J. M. Kuypers & R. A. Proctor, 1989, 57, 2306–2312). PCR and Southern hybridization analysis with primers and probes, respectively, for the and genes of strain 8325–4 showed that strain 879R4SSp possesses a single gene which is homologous to This was confirmed by sequencing 41 bp of 5′ non-coding and 237 bp of 5′ coding DNA, which showed 97% base identity to Southern hybridization and sequencing showed that Tn was inserted 41 bp 5′ to in the mutant 879R4SSp/1536, between the promoter and initiation codon. Reduced adherence of the mutant to surface-bound fibronectin correlated with lower expression of a 180 kDa wall-associated fibronectin-binding protein.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2153
1996-08-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2153.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2153&mimeType=html&fmt=ahah

References

  1. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Smith J.A., Seidman J.J., Struhl K. 1987 Current Protocols in Molecular Biology. New York: John Wiley;
    [Google Scholar]
  2. Boden M.K., Flock J.-I. 1989; Fibrinogen-binding protein/clumping factor from Staphylococcus aureus . Infect Immun 57:2358–2363
    [Google Scholar]
  3. Bozzini S., Visai L., Pignatti P., Petersen T.E., Speziale P. 1992; Multiple binding sites in fibronectin and the staphylococcal fibronectin receptor. Eur J Biochem 207:327–333
    [Google Scholar]
  4. Chhatwal G.S., Preissner K.T., Muller-Berghaus G., Blobel H. 1987; Specific binding of the human S protein (vitronectin) to streptococci, Staphylococcus aureus and Escherichia coli . Infect Immun 55:1878–1883
    [Google Scholar]
  5. Clewell D.B., An F.Y., White B.A., Gawron-Burke C. 1985; Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918). J Bacterial 162:1212–1220
    [Google Scholar]
  6. Clewell D.B., Flannagan S.E., Ike Y., Jones J.M., Gawron-Burke C. 1988; Sequence analysis of termini of conjugative transposon Tn916 . J Bacterial 170:3046–3052
    [Google Scholar]
  7. Greene C., McDevitt D., Francois P., Vaudaux P., Lew D.P., Foster T.J. 1995; Adhesion properties of mutants of Staphylococcus aureus defective in fibronectin-binding proteins and studies on expression of fnb genes. Mol Microbiol 17:1143–1152
    [Google Scholar]
  8. Hanski E., Caparon M.G. 1992; Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes . Proc Natl Mead Sci USA 896172–6176
    [Google Scholar]
  9. Hawley D.K., McClure W.R. 1983; Compilation and analysis of Escherichia coli promoter sequences. Nucleic Acids Res 11:2237–2255
    [Google Scholar]
  10. Holderbaum D., Spech T., Ehrhart L.A., Keys T., Hall G.S. 1987; Collagen binding in clinical isolates of Staphylococcus aureus . J Clin Microbiol 25:2258–2261
    [Google Scholar]
  11. Jonsson K., Signas C., Muller H.-P., Lindberg M. 1991; Two different genes encode fibronectin-binding proteins in Staphylococcus aureus. The complete nucleotide sequence and characterization of the second gene. Eur J Biochem 202:1041–1048
    [Google Scholar]
  12. Kuypers J.M., Proctor R.A. 1989; Reduced adherence to traumatized rat heart valves by a low-fibronectin-binding mutant of Staphylococcus aureus . Infect Immun 57:2306–2312
    [Google Scholar]
  13. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  14. Lindberg M., Sjostrom J.E., Johansson J. 1972; Transformation of chromosomal and plasmid characters in Staphylococcus aureus . J Bacteriol 109:844–847
    [Google Scholar]
  15. Lindgren P.-E., Speziale P., McGavin M., Monstein H.J., Hook M., Visai L., Kostiainen T., Bozzini S., Lindberg M. 1992; Cloning and expression of two different genes from Streptococcus dysgalactiae encoding fibronectin receptors. J Biol Chem 267:1924–1931
    [Google Scholar]
  16. Lopes J.D., dos Reis M., Brentani R. R. 1985; Presence of laminin receptors in Staphylococcus aureus . Science 229:275–277
    [Google Scholar]
  17. McDevitt D., Francois P., Vaudaux P., Foster T.J. 1994; Molecular characterization of the fibrinogen receptor (clumping factor) of Staphylococcus aureus . Mol Microbiol 11:237–248
    [Google Scholar]
  18. McGavin M.J., Raucci G., Gurusiddappa S., Hook M. 1991; Fibronectin-binding determinants of the Staphylococcus aureus fibronectin receptor. J Biol Chem 266:8343–8347
    [Google Scholar]
  19. McGavin M.J., Gurusiddappa S., Lindgren P.-E., Lindberg M., Raucci G., Hook M. 1993; Fibronectin receptors from Streptococcus dysgalactiae and Staphylococcus aureus. Involvement of conserved residues in ligand binding. J Biol Chem 268:23946–23953
    [Google Scholar]
  20. Maxe I., Ryden C., Wadstrom T., Rubin K. 1987; Specific attachment of Staphylococcus aureus to immobilized fibronectin. Infect Immun 54:695–704
    [Google Scholar]
  21. Miller J.H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  22. Moreillon P., Entenza J.M., Francioli P., McDevitt D., Foster T.J., Francois P., Vaudaux P. 1995; Role of Staphylococcus aureus coagulase and clumping factor in the pathogenesis of experimental endocarditis. Infect Immun 63:4738–4743
    [Google Scholar]
  23. Navarre W.W., Schneewind O. 1994; Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in Gram positive bacteria. Mol Microbiol 14:115–121
    [Google Scholar]
  24. Novick R.P. 1963; Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus . Virology 33:155–166
    [Google Scholar]
  25. Raja R.H, Raucci C., Hook M. 1990; Peptide analogs to a fibronectin receptor inhibit attachment of Staphylococcus aureus to fibronectin-coated substrates. Infect Immun 58:2593–2598
    [Google Scholar]
  26. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  27. Santoro J., Levison M.W. 1978; Rat model of experimental endocarditis. Infect Immun 19:915–918
    [Google Scholar]
  28. Schneewind O., Mihaylova-Petkov D., Model P. 1993; Cell wall sorting signals in surface proteins of Gram-positive bacteria. EMBO J 12:4803–4811
    [Google Scholar]
  29. Signas C., Raucci G., Jonsson K., Lindgren P.-E., Anantharamaiah G.M., Hook M., Lindberg M. 1989; Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptides. Proc Natl Acad Sci USA 86699–703
    [Google Scholar]
  30. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
    [Google Scholar]
  31. Speziale P., Raucci G., Visai L., Switalski L.M., Timpl R., Hook M. 1986; Binding of collagen to Staphylococcus aureus Cowan 1. J Bacteriol 167:77–81
    [Google Scholar]
  32. Vaudaux P., Waldvogel F.A., Morgenthaler J.J., Nydegger U.E. 1984; Adsorption of fibronectin onto polymethylmethacrylate and promotion of Staphylococcus aureus adhesion. Infect Immun 45:768–774
    [Google Scholar]
  33. Vaudaux P., Pittet D., Haeberli A., Huggler E., Nydegger U.E., Lew D.P., Waldvogel F.A. 1989; Host factors selectively increase staphylococcal adherence on inserted catheters: a role for fibronectin and fibrinogen or fibrin. J Infect Dis 160:865–875
    [Google Scholar]
  34. Vaudaux P., Pittet D., Haeberli A., Lerch P.G., Morgenthaler J.J., Proctor R.A., Waldvogel F.A., Lew D.P. 1993; Fibronectin is more active than fibrin or fibrinogen in promoting Staphylococcus aureus adherence to inserted intravascular devices. J Infect Dis 167:633–641
    [Google Scholar]
  35. Vaudaux P.E., Francois P., Proctor R.A., McDevitt D., Foster T.J., Albrecht R.M., Lew D.P., Wabers H., Cooper S.L. 1995; Use of adhesion-defective mutants of Staphylococcus aureus to define the role of specific plasma proteins in promoting bacterial adhesion to canine arteriovenous shunts. Infect Immun 63:585–590
    [Google Scholar]
  36. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2153
Loading
/content/journal/micro/10.1099/13500872-142-8-2153
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error