1887

Abstract

The non-haem chloroperoxidase gene () from the pyrrolnitrin producer BL914 was cloned using an oligonucleotide derived from part of the N-terminal amino acid sequence of chloroperoxidase (CPO-P) from as a probe. Based on the overexpression of in and the stabilty of CPO-F against higher temperatures and proteases, the enzyme was purified to homogeneity. Partial characterization of the enzyme showed that it belongs to the class of bacterial non-haem CPOs. To investigate the role of CPO-F in pyrrolnitrin biosynthesis, the gene was inactivated by insertion of a kanamycin cassette. Exchange of the chromosomal gene against the disrupted copy had no influence on pyrrolnitrin production demonstrating that CPO-F was not involved in pyrrolnitrin biosynthesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2129
1996-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2129.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2129&mimeType=html&fmt=ahah

References

  1. Arima K., Imanaka H., Kousaka M., Fukuda A., Tamura G. 1964; Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas . Agr Biol Chem 28:575–576
    [Google Scholar]
  2. Bantleon R., Altenbuchner J., van Pée K.-H. 1994; Chloro-peroxidase from Streptomyces lividans: isolation and characterization of the enzyme and the corresponding gene. J Bacterial 176:2339–2347
    [Google Scholar]
  3. Bolivar F., Rodriguez R.L., Green P.J., Betlach M.C., Heyneker H.L., Boyer H.W., Crosa J.H., Falkow S. 1977; Construction and characterization of a new cloning vehicle. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  4. Bongs G., van Pée K.-H. 1994; Enzymatic chlorination using bacterial nonheme haloperoxidases. Enɀyme Microb Technol 16:53–60
    [Google Scholar]
  5. Burd W., Yourkevich O., Voskoboev A.J., van Pɀe K.-H. 1995; Purification and properties of a non-haem chloroperoxidase from Serratia marcescens . FEMS Microbiol Lett 129:255–260
    [Google Scholar]
  6. Dairi T., Nakano T., Aisaka K., Katsumata R., Hasegawa M. 1995; Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline. Biosci Biotech Biochem 59:1099–1106
    [Google Scholar]
  7. Davis R.W., Botstein D., Roth J.R. 1980 A Manual for Genetic Engineering. Advanced Bacterial Genetics. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  8. Duggar B. M. 1948; Aureomycin, a product of the continuing search for new antibiotics. Ann N Y Acad Sci 51:171–181
    [Google Scholar]
  9. Ehrlich J., Bartz Q.R., Smith R.M., Joslyn D.A., Burkholder P.R. 1947; Chloromycetin, a new antibiotic from soil Actinomycete . Science 106:417
    [Google Scholar]
  10. Elander R.P., Mabe J.A., Hamill R.H., Gorman M. 1968; Metabolism of tryptophan by Pseudomonas aureofaciens. VI. Production of pyrrolnitrin by selected Pseudomonas species. Appl Microbiol 16:753–758
    [Google Scholar]
  11. Facey S.J., Groᵦ F., Vining L.C., Yang K., van Pée K.-H. 1996; Cloning, sequencing and disruption of a bromoperoxidase-catalase gene in Streptomyces veneɀuelae: evidence that it is not required for chlorination in chloramphenicol biosynthesis. Microbiology 142:657–665
    [Google Scholar]
  12. Gaffney T.D., Lam S.T., Ligon J., Gates K., Frazelle A., Di Maio J., Hill S., Goodwin S., Torkewitz N., Allshouse A.M., Kempf H.-J., Becker J.O. 1994; Global regulation of expression of antifungal factors by a Pseudomonas fluorescens biological control strain. Mol Plant-Microbe Interact 7:455–463
    [Google Scholar]
  13. Gibson T.J. 1984 Studies on the Epstein-Barr virus genome. PhD thesis University of Cambridge, UK.:
    [Google Scholar]
  14. Haag T., Lingens F., van Pée K.-H. 1991; A metal-ion- and cofactor-independent enzymatic redox reaction: halogenation by bacterial nonheme haloperoxidases. Angew Chem Int Ed Engl 30:1487–1488
    [Google Scholar]
  15. Hager L.P., Morris D.R., Brown F.S., Eberwein H. 1966; Chloroperoxidase. II. Utilization of halogen anions. J Biol Chem 241:1769–1777
    [Google Scholar]
  16. Hecht H.J., Sobek H., Haag T., Pfeifer O., van Pée K.-H. 1994; The metal-ion-free oxidoreductase from Streptomyces aureofaciens has an α/β hydrolase fold. Nature Struct Biol 1:532–537
    [Google Scholar]
  17. Hill S.D., Stein J.I., Torkewitz N.R., Morse A.M., Howell C.R., Pachlatko J.P., Becker J.O., Ligon J.M. 1994; Cloning of genes involved in the synthesis of pyrrolnitrin from Pseudomonas fluorescens and role of pyrrolnitrin synthesis in biological control of plant disease. Appl Environ Microbiol 60:78–85
    [Google Scholar]
  18. Imanaka H., Kousaka M., Tamura G., Arima K. 1965; Studies on pyrrolnitrin, a new antibiotic. II. Taxonomic studies on pyrrolnitrin-producing strain. J Antibiot (Tokyo) Ser A 18:205–206
    [Google Scholar]
  19. Itoh N., Morinaga N., Kouzai T. 1993; Oxidation of aniline to nitrobenzene by nonheme bromoperoxidase. Biochem Mol Biol Int 29:5194–5200
    [Google Scholar]
  20. Kirner S., van Pée K.-H. 1994; The biosynthesis of nitro compounds: the enzymatic oxidation to pyrrolnitrin of its amino-substituted precursor. Angew Chem Int Ed Engl 33:352
    [Google Scholar]
  21. Maurer H.R. 1964 Disk-Elektrophorese: Theorie und Praxis der diskontinuierlichen Polyacrylamidgelelektrophorese. Berlin, New York:: Walter de Gruyter Verlag.;
    [Google Scholar]
  22. McCormick M.H., Stark W.M., Pittenger G.E., Pittenger R.C., MacGuire J.M. 1956; Vancomycin, a new antibiotic. I. Chemical and biological properties. Antibiot Annu 1955/56:606–611
    [Google Scholar]
  23. Neidleman S.L., Geigert J. 1986 Biohalogenation: Principles, Basic Roles and Applications. Chichester:: Ellis Horwood Ltd.;
    [Google Scholar]
  24. van Pée K.-H. 1988; Molecular cloning and high-level expression of a bromoperoxidase gene from Streptomyces aureofaciens Tü24. J Bactenol 170:5890–5894
    [Google Scholar]
  25. van Pée K.-H. 1990; Bacterial haloperoxidases and their role in secondary metabolism. Biotech Adv 8:185–205
    [Google Scholar]
  26. van Pée K.-H., Salcher O., Lingens F. 1980; Formation of pyrrolnitrin and 3-(2-amino-3-chlorophenyl)pyrrole from 7-chloro-tryptophan. Angew Chem Int Ed Engl 19:828
    [Google Scholar]
  27. van Pée K.-H., Hecht H.J., Berkessel A., Schrapel T., Laatsch H. 1994 Enɀymatische, aktiven Sauerstojf liefernde Mischung sowie Verfahren ɀur enɀymatischen Herstellung von Persäuren. German patent No P 44:327.0.
    [Google Scholar]
  28. Pelletier I., Pfeifer O., Altenbuchner J., van Pée K.-H. 1994; Cloning of a second non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762: sequence analysis, expression in Streptomyces lividans and enzyme purification. Microbiology 140:509–516
    [Google Scholar]
  29. Pfeifer O., Pelletier I., Altenbuchner J., van Pée K.-H. 1992; Molecular cloning and sequencing of a non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762. J Gen Microbiol 138:1123–1131
    [Google Scholar]
  30. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  31. Schägger H., von Jagow G. 1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379
    [Google Scholar]
  32. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1:784–790
    [Google Scholar]
  33. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
    [Google Scholar]
  34. Stahl E., Kaldewey H. 1961; Spurenanalyse physiologisch aktiver, einfacher Indolderivate. Hoppe-Seyler̓s Z Physiol Chem 323:182–191
    [Google Scholar]
  35. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  36. Vilter H. 1984; Peroxidases from Phyophyceae: a vanadium (V)-dependent peroxidase from Ascophyllum nodosum . Phytochem 23:1387–1390
    [Google Scholar]
  37. Wever R., Plat H., de Boer E. 1985; Isolation procedure and some properties of the bromoperoxidase from the seaweed Ascophyllum nodosum . Biochim Biophys Acta 830:181–186
    [Google Scholar]
  38. Wiesner W., van Pée K.-H., Lingens F. 1988; Purification and characterization of a novel bacterial non-heme chloroperoxidase from Pseudomonas pyrrocinia . J Biol Chem 263:13725–13732
    [Google Scholar]
  39. Whitaker J.R., Granum P.E. 1980; An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Anal Biochem 109:156–159
    [Google Scholar]
  40. Wolfframm C., van Pée K.-H., Lingens F. 1988; Cloning and high-level expression of a chloroperoxidase gene from Pseudomonas pyrrocinia in Escherichia coli . FEBS Lett 238:325–328
    [Google Scholar]
  41. Zeiner R., van Pée K.-H., Lingens F. 1988; Purification and partial characterization of multiple bromoperoxidases from Streptomyces griseus . J Gen Microbiol 134:3141–3149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2129
Loading
/content/journal/micro/10.1099/13500872-142-8-2129
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error