1887

Abstract

The outer membrane of a cyanobacterium ( sp. strain PCC 7942) contains only a few major proteins. A gene encoding one of them, , was cloned and characterized. Based on the nucleotide sequence, SomA was predicted to comprise 531 amino acids with a calculated molecular mass of 57136 Da. The deduced amino acid sequence of SomA shares similarities with two bacterial cell-surface proteins, the S-layer protein of and the flagellin of The predicted amino acid sequence of SomA revealed also that it contains a signal peptide-like sequence at its N terminus. This signal peptide-like sequence was capable of mediating protein translocation across the cytoplasmic membrane into the outer membrane of , provided that this sequence was fused to the outer-membrane protein, OmpF. The signal peptide-like sequence was cleaved upon the translocation of the SomA::OmpF protein. We suggest that SomA is synthesized as a precursor and that its N-terminal 24 amino acid sequence is a cleavable signal peptide involved in protein targeting into the outer membrane. To our knowledge, this is the first example of cleavable signal peptides for proteins transported into the outer membrane of cyanobacteria.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-2121
1996-08-01
2021-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-2121.html?itemId=/content/journal/micro/10.1099/13500872-142-8-2121&mimeType=html&fmt=ahah

References

  1. Barbrook A.C., Packer J.C., Howe C.J. 1993; Components of the protein translocation machinery in the thermophilic cyanobacterium Phormidium laminosum . Biochem Biophys Res Commun 197:874–877
    [Google Scholar]
  2. Briggs L.M., Pecoraro V.L., Mclntosh L. 1990; Copper- induced expression, cloning, and regulatory studies of the plasto- cyanin gene from the cyanobacterium Synechocystis sp. PCC6803. Plant Mol Biol 15:633–642
    [Google Scholar]
  3. Chaddock A.M., Mant A., Karnauchov I., Brink S., Herrman R.G., Klösgen R.B., Robinson C. 1995; A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the △pH-dependent thylakoidal translocase. EMBO J 14:2715–2722
    [Google Scholar]
  4. Chitnis P.R., Reilly P.A., Miede M., Nelson N. 1989; Structure and targeted mutagenesis of the gene encoding 8-kDa subunit of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem 264:18374–18380
    [Google Scholar]
  5. Datta D.B., Arden B., Henning U. 1977; Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors. J Bacteriol 131:821–829
    [Google Scholar]
  6. Faraldo M.M., Pedro M.A., Berenguer J. 1992; Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli . J Bacteriol 174:7458–7462
    [Google Scholar]
  7. Filip C., Fletcher G.., Wulff J. L., Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol 115:717–722
    [Google Scholar]
  8. Fischer K., Weber A., Brink S., Arbinger B., Schunemann D., Borchert S., Heldt H.W., Popp B., Benz R., Link T. A. 1994; Porins from plants. Molecular cloning and functional characterization of two new members of the porin family. J Biol Chem 269:25754–25760
    [Google Scholar]
  9. Guerry P., Alm R.A., Power M.E., Logan S.M., Trust T.J. 1991; Role of two flagellin genes in Campylobacter motility. J Bacteriol 173:4757–4764
    [Google Scholar]
  10. Hansel A., Schmid A., Tadros M.H., Jurgens U.J. 1994; Isolation and characterization of porin from the outer membrane of Synechococcus PCC 6301. Arch Microbiol 161:163–167
    [Google Scholar]
  11. von Heijne G. 1994; Signals for protein targeting into and across membranes. Subcell Biochem 22:1–19
    [Google Scholar]
  12. Kuwabara T., Reddy K.J., Sherman L.A. 1987; Nucleotide sequence of the gene from the cyanobacterium Anacystis nidulans R2 encoding the Mn-stabilizing protein involved in photosystem II water oxidation. Proc Natl Acad Sci USA 848230–8234
    [Google Scholar]
  13. Laudenbach D.E., Herbert S.K., McDowell C., Fork D.C., Grossman A.R., Straus N.A. 1990; Cytochrome f-553 is not required for photosynthetic activity in the cyanobacterium Synechococcus . Plant Cell 2:913–924
    [Google Scholar]
  14. Laudenbach D.E., Ehrhardt D., Green L., Grossman A. 1991; Isolation and characterization of a sulfur-regulated gene encoding a periplasmically localized protein with sequence similarity to rho- danese. J Bacteriol 173:2751–2760
    [Google Scholar]
  15. Mackle M.M., Zilinskas B.A. 1994; Role of signal peptides in targeting of proteins in cyanobacteria. J Bacteriol 176:1857–1864
    [Google Scholar]
  16. Masui Y., Mizuno T., Inouye M. 1984; Novel high-level expression cloning vehicles: 104-fold amplification of Escherichia coli minor protein. Bio/Technology 3:81–85
    [Google Scholar]
  17. Matsuyama S., Inokuchi K., Mizushima S. 1984; Promoter exchange between ompF and ompC, genes for osmoregulated major outer membrane proteins of Escherichia coli K-12. J Bacteriol 158:1041–1047
    [Google Scholar]
  18. Mizuno T., Mizushima S. 1987; Isolation and characterization of deletion mutants of ompR and envZ, regulatory genes for expression of the outer membrane proteins OmpC and OmpF in Escherichia coli . J Biochem 101:387–396
    [Google Scholar]
  19. Mizuno T., Mizushima S. 1990; Signal transduction and gene regulation through the phosphorylation of two regulatory components : the molecular basis for the osmotic regulation of the porin gene. Mol Microbiol 4:1077–1082
    [Google Scholar]
  20. Mizuno T., Chou M.-Y., Inouye M. 1983; A comparative study on the genes for three porins of the Escherichia coli outer membrane: DNA sequence of the osmoregulated ompC gene. J Biol Chem 258:6932–6940
    [Google Scholar]
  21. Murata N., Omata T. 1988; Isolation of cyanobacterial plasma membranes. Methods Engymol 167:245–251
    [Google Scholar]
  22. Nakai M., Tanaka A., Omata T., Endo T. 1992; Cloning and characterization of the secY gene from the cyanobacterium Synechococcus PCC7942. Biochim Biophys Acta 1171:113–116
    [Google Scholar]
  23. Nakai M., Nohara T., Sugita D., Endo T. 1994; Identification and characterization of the SecA protein homologue in the cyanobacterium Synechococcus PCC7942. Biochem Biophys Res Commun 200:844–851
    [Google Scholar]
  24. Nikaido H. 1979; Nonspecific transport through the outer membrane. In Bacterial Outer Membrane: Biogenesis and Function pp. 319–361 Edited by Inouye M. New York: John Wiley and Sons;
    [Google Scholar]
  25. Packer J.C, Andre D., Howe C.J. 1995; Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum . Plant Mol Biol 27:199–204
    [Google Scholar]
  26. Philbrick J.B., Zilinskas B.A. 1988; Cloning, nucleotide sequence and mutational analysis of the gene encoding the Photosystem II manganese-stabilizing polypeptide of Sjnechocjstis 6803. Mol Gen Genet 212:418–425
    [Google Scholar]
  27. Pugsley A.P. 1993; The complete general secretory pathway in Gram-negative bacteria. Microbiol Ren 57:50–108
    [Google Scholar]
  28. Rippka R., Deruelles J., Waterbury j.B., Herdman M., Stanier R.Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
    [Google Scholar]
  29. Shestakov S.V., Anbudurai P.R., Stanbekova G.E., Gadzhiev A., Lind L.K., Pakrasi H.B. 1994; Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease. Analysis of a spontaneous photosystem II- deficient mutant strain of the cyanobacterium Sjnechocjstis sp. PCC6803. J Biol Chem 269:19354–19359
    [Google Scholar]
  30. Snyder M., Davis R.W. 1985; Screening zgtll expression libraries with antibody probes. In Hjbridoma Technology in Biosciences and Medicine pp. 397–406 Edited by Springer T. New York: Plenum Press;
    [Google Scholar]
  31. Suzuki T., Itoh A., Ichihara S., Mizushima S. 1987; Characterization of the sppA gene coding for protease IV, a signal peptide peptidase of Escherichia coli . J Bacterial 169:2523–2528
    [Google Scholar]
  32. Varley J.P., Moehrle J.J., Manasse R.S., Bendall D.S., Howe C.J. 1995; Characterization of plastocyanin from the cyanobacterium Phormidiutn laminosum-. copper-inducible expression and SecA-dependent targeting in Escherichia coli . Plant Mol Biol 27:179–190
    [Google Scholar]
  33. Vieira J., Messing J. 1987; Production of single stranded plasmid DNA. Methods Enzymol 153:3–11
    [Google Scholar]
  34. Zhang C.-C., Durand M.-C., Jeanjean R., Joset F. 1989; Molecular and genetical analysis of the fructose-glucose transport system in the cyanobacterium Sjnechocjstis PCC6803. Mol Microbiol 3:1221–1229
    [Google Scholar]
  35. Zhang L., Pakrasi H.B., Whitmarsh J. 1994; Photoautotrophic growth of the cyanobacterium Sjnechocjstis sp. PCC6803 in the absence of cytochrome r553 and plastocyanin. J Biol Chem 269:5036–5042
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-2121
Loading
/content/journal/micro/10.1099/13500872-142-8-2121
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error