The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in S-313 Free

Abstract

Conditions were optimized for the batch growth of S-313 under sulfur-limited conditions. grew exponentially with sulfate as the sole source of sulfur, and growth was concomitant with the utilization of sulfate until it was exhausted. A further 20% of protein was synthesized after the apparent disappearance of sulfate. A mass balance for the utilized sulfate in cell material was calculated, given the observed molar growth yield of about 3·6 kg protein (mol S) and a sulfur content of 0·41% S in dry matter. Similar data were obtained for growth with cysteine and thiocyanate. The organism also grew exponentially with 4-toluenesulfonate (TS) as sulfur source, essentially as observed with sulfate, except that negligible protein formation after exhaustion of TS was observed. Similar data were also obtained with 4-nitrocatecholsulfate (NCS) and ethanesulfonate. Any substrate pair selected from sulfate, cysteine and thiocyanate was utilized simultaneously, and although one of the pair of substrates was always preferred, growth continued at the same rate when only one substrate remained. Growth after substrate exhaustion was observed. Any substrate pair selected from TS, NCS and ethanesulfonate gave similar data, but with less growth after exhaustion of the sulfur sources. If a mixed substrate pair was chosen from the two groups, the sulfur source from the first-named group was initially used exclusively, and the second source of sulfur was utilized subsequently, after a lag phase. The data are considered to reflect the control of scavenging for sulfur and of distribution of sulfur in the cell exerted by the sulfate-starvation-induced stimulon [Kertesz, Leisinger & Cook, (1993) 175, 1187-1189].

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-1989
1996-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-1989.html?itemId=/content/journal/micro/10.1099/13500872-142-8-1989&mimeType=html&fmt=ahah

References

  1. Autry A.R., Fitzgerald J.W. 1990; Sulfonate S: a major form of forest soil organic sulfur. Biol Fertil Soils 10:50–56
    [Google Scholar]
  2. Beil S., Kehrli H., James P., Staudemann W., Cook A.M., Leisinger T., Kertesz M.A. 1995; Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium, and cloning of the arylsulfatase gene ( atsA). . Fur J Biochem 229:385–394
    [Google Scholar]
  3. Busse H.-J., El-Banna T., Auling G. 1989; Evaluation of different approaches for identification of xenobiotic-degrading pseudomonads. Appl Environ Microbiol 55:1578–1583
    [Google Scholar]
  4. Dodgson K.S., White G.F., Fitzgerald J.W. 1982 Sulfatasesof Microbial Origin Boca Raton: CRC Press;
    [Google Scholar]
  5. Fariss M.W., Reed D. J. 1987; High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives. A lethods Ensymol 143:101–109
    [Google Scholar]
  6. Fitzgerald J.W. 1978; Naturally occurring organosulfur com¬pounds in soil. In Sulfur in the Environment pp. 391–443 Edited by Nriagu J.O. New York: Wiley;
    [Google Scholar]
  7. Harder W., Dijkhuizen L. 1983; Physiological responses to nutrient limitation. Annu Rev Microbiol 37:1–23
    [Google Scholar]
  8. Harvey N.L., Fewson C.A., Holms W.H. 1968; Apparatus for batch culture of micro-organisms. Lab Pract 17:1134–1136
    [Google Scholar]
  9. Kayser K.J., Bielaga-Jones B.A., Jackowski K., Odusan O., Kilbane J.J. 1993; Utilization of organosulfur compounds by axenic and mixed cultures of Rhodococcus rhodochrous IGTS8. J Gen Microbiol 139:3123–3129
    [Google Scholar]
  10. Kennedy S.I.T., Fewson C.A. 1968; Enzymes of the mandelate pathway in bacterium N.C.I.B.8250. Biochem J 107:497–506
    [Google Scholar]
  11. Kertesz MA., Leisinger T., Cook A.M. 1993; Proteins induced by sulfate limitation in Escherichia coli , Pseudomonas putida, or Staphylococcus aureus. . J Bacteriol 175:1187–1190
    [Google Scholar]
  12. Kertesz M.A., Cook A.M., Leisinger T. 1994a; Microbial metabolism of sulfur- and phosphorus-containing xenobiotics and its regulation. FEMS Microbiol Rev 15:195–215
    [Google Scholar]
  13. Kertesz M.A., Kölbener P., Stockinger H., Beil S., Cook A.M. 1994b; Desulfonation of linear alkylbenzenesulfonate (LAS) surfactants and related compounds by bacteria. Appl Environ Microbiol 602296–2303
    [Google Scholar]
  14. Kredich N.M. 1992; The molecular basis for positive regulation of cys promotors in Salmonella typhimurium and Escherichia coli. . Mol Microbiol 6:2747–2753
    [Google Scholar]
  15. Luria S.E. 1960; The bacterial protoplasm: composition and organization. In The Bacteria 1: pp. 1–34
    [Google Scholar]
  16. Mason C.A., Egli T. 1993; Dynamics of microbial growth in the decelerating and stationary phase of batch culture. In Starvation in Bacteria pp. 81–102 Edited by Kjelleberg S. New York: Plenum Press;
    [Google Scholar]
  17. Mazel D., Marlière re. 1989; Adaptive eradication of methionine and cysteine from bacterial light-harvesting proteins. Nature 341:245–248
    [Google Scholar]
  18. Neidhardt F.C. 1987; Multigene systems and regulons. In Escherichica coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 1313–1317 Edited by Neidhardt F.C., Ingraham J.L., Brooks Low K., Magasanik B., Schaechter M., Umbarger H.E. Washington: American Society for Microbiology;
    [Google Scholar]
  19. Omori T., Monna L., Saiki Y., Kodama T. 1992; Desulfuration of dibenzothiophene by Corynebacterium sp.strain SY1. Appl Environ Microbiol 58:911–915
    [Google Scholar]
  20. Reed D.J., Babson J.R., Beatty P.W., Brodie A.E., Ellis W.W., Potter D.W. 1980; High performance liquid chromatographic analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides. Anal Biochem 106:55–62
    [Google Scholar]
  21. Roberts R.B., Abelson P.H., Cowie D.B., Bolton E.T., Britten R.J. 1955; Sulfur metabolism. In Studies of Biosynthesis in Escherichia coli pp. 318–405 Edited by Roberts R.B., Cowie D.B., Abelson P.H., Bolton E.T., Britten R.J. Washington, DC: Carnegie Institute of Washington;
    [Google Scholar]
  22. Seitz A.P., Leadbetter E.R., Godchaux W. III 1993; Utilization of sulfonates as sole sulfur source by soil bacteria including Comamonas acidovorans. . Arch Microbiol 159:440–444
    [Google Scholar]
  23. Sörbo B.H. 1955; Rhodanese. Methods Enzymol 2:334–337
    [Google Scholar]
  24. Thurnheer T., Köhler T., Cook A.M., Leisinger T. 1986; Orthanilic acid and analogues as carbon sources for bacteria: growth physiology and enzymic desulfonation. J Gen Microbiol 132:1215–1220
    [Google Scholar]
  25. Thurnheer T., Zürrer D., Höglinger O., Leisinger T., Cook A.M. 1990; Initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acid and orthanilic acid in Alcaligenes sp.strain O-l. Biodegradation 1:54–63
    [Google Scholar]
  26. Uria-Nickelsen M.R., Leadbetter E.R., Godchaux W. III 1993a; Sulfonate utilization bv enteric bacteria. J Gen Microbiol 139:203–208
    [Google Scholar]
  27. Uria-Nickelsen M.R., Leadbetter E.R., Godchaux W. III 1993b; Sulfonate-sulfur assimilation by yeasts resembles that of bacteria. FEMS Microbiol Eett 114:73–78
    [Google Scholar]
  28. Wanner U., Egli T. 1990; Dynamics of bacterial growth and cell composition in batch culture. FEMS Microbiol Rev 75:19–44
    [Google Scholar]
  29. Ward D.M., Winfrey M.R. 1985; Interactions between methanogenic and sulfate-reducing bacteria in sediments. Adv Aquat Microbiol 3:141–179
    [Google Scholar]
  30. Zürrer D., Cook A.M., Leisinger T. 1987; Microbial desulfonation of substituted naphthalenesulfonic acids and benzene- sulfonic acids. Appl Environ Microbiol 53:1459–1463
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-1989
Loading
/content/journal/micro/10.1099/13500872-142-8-1989
Loading

Data & Media loading...

Most cited Most Cited RSS feed