1887

Abstract

The influence of CO on the ability of U-1 (ATCC 35244) to use an aromatic acrylate group as an energy-conserving electron acceptor during -methyl-dependent growth was examined. Ferulate (a methoxylated phenylacrylate), unlike hydroferulate (a methoxylated phenylpropionate),supported growth under CO-limited conditions. Two phases occurred during ferulate utilization in CO-limited cultures. In phase I (maximum growth), -methyl-derived reductant was coupled mainly to acrylate group reduction, and acetate synthesis (CO as reductant sink) was minimal. In phase II, acetate synthesis increased, but cell yields in this phase were much less than in phase I. In CO-enriched cultures, distinct phases were not observed; reductant was coupled equally to CO and acrylate group reduction. Under CO-enriched conditions, -methyl and acrylate groups were incompletely metabolized, and molar growth yields were significantly lower compared to CO-limited conditions. Resting cell studies indicated that -demethylase and aromatic acrylate oxidoreductase activities were induced by ferulate. These findings demonstrated that U-1 can use the aromatic acrylate oxidoreductase system as a sole, energy-conserving, electron-accepting process, but is not able to prevent the simultaneous use of the bioenergetically less favourable acetyl-CoA pathway during -methyl-dependent growth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-8-1983
1996-08-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/8/mic-142-8-1983.html?itemId=/content/journal/micro/10.1099/13500872-142-8-1983&mimeType=html&fmt=ahah

References

  1. Bache R., Pfennig N. 1981; Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields. Arch Microbiol 130:255–261
    [Google Scholar]
  2. Beaty P.S., Ljungdahl L.G. 1990; Thiosulfate reduction by Clostridium thermoaceticum and Clostridium thermoautotrophicum during growth on methanol. In Abstracts of the 90th Annual Meeting of the American Society for Microbiology abstr. I-7 p. 199 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Cord-Ruwisch R., Seitz H.-J., Conrad R. 1988; The capacity of hydrogenotrophic anaerobic bacteria to compete for traces of hydrogen depends on the redox potential of the terminal electron acceptor. Arch Microbiol 149:350–357
    [Google Scholar]
  4. Daniel S.L., Hsu T., Dean S.I., Drake H.L. 1990; Characterization of the H2− and CO-dependent chemolithotrophic potentials of the acetogens Clostridium thermoaceticum and Acetogenium kivui . J Bacteriol 172:4464–4471
    [Google Scholar]
  5. Daniel S.L., Keith E.S., Yang H., Lin Y.-S., Drake H.L. 1991; Utilization of methoxylated aromatic compounds by the acetogen Clostridium thermoaceticum: expression and specificity of the CO-dependent O-demethylating activity. Biochem Biophys Res Commun 180:416–422
    [Google Scholar]
  6. Diekert G., Wohlfarth G. 1994; Metabolism of homoacetogens. Antonie Leeuwenhoek 66:209–221
    [Google Scholar]
  7. Drake H.L. 1994; Acetogenesis, acetogenic bacteria, and the acetyl-CoA “Wood/Ljungdahl” pathway: past and current perspectives. In Acetogenesis pp. 3–60 Edited by Drake L.Drake. New York: Chapman & Hall;
    [Google Scholar]
  8. Drake H.L., Daniel S.L., Matthies C., Küsel K. 1994; Acetogenesis: reality in the laboratory, uncertainty elsewhere. In Acetogenesis pp. 273–302 Edited by Drake L.Drake. New York: Chapman & Hall;
    [Google Scholar]
  9. Frazer A.C. 1994; O-Demethylation and other transformations of aromatic compounds by acetogenic bacteria. In Acetogenesis pp. 445–483 Edited by Drake H.L. New York: Chapman & Hall;
    [Google Scholar]
  10. Frazer A.C., Young L.Y. 1985; A gram-negative anaerobic bacterium that utilizes O-methyl substituents of aromatic acids. Appl Environ Microbiol 49:1345–1347
    [Google Scholar]
  11. Fröstl J.M., Drake H.L. 1994; Dissimilatory reduction of nitrate by the acetogen Clostridium thermoautotrophicum . In Abstracts of the 94th General Meeting of the American Society for Microbiology abstr.K-22 p. 279 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Hansen B., Bokranz M., Schönheit P., Kröger A. 1988; ATP formation coupled to caffeate reduction by H2 in Acetobacterium woodii NZva16. Arch Microbiol 150:447–451
    [Google Scholar]
  13. Kasmi A.E., Rajasekharan S., Ragsdale S. 1994; Anaerobic pathway for conversion of the methyl group of aromatic methyl ethers to acetic acid by Clostridium thermoaceticum . Biochemistry 33:11217–11224
    [Google Scholar]
  14. Krumholz L.R., Bryant M.P. 1985; Clostridiump fennigii sp.nov.uses methoxyl groups of monobenzenoids and produces butyrate. Int J Syst Bacteriol 35:454–456
    [Google Scholar]
  15. Krumholz L.R., Bryant M.P. 1986; Syntrophococcus sucromutans sp.nov.gen.nov.uses carbohydrates as electron donors and formate, methoxymonobenzenoids or Methanobrevibacter as electron acceptor systems. Arch Microbiol 143:313–318
    [Google Scholar]
  16. Ljungdahl L.G. 1994; The acetyl-CoA pathway and the chemiosmotic generation of ATP during acetogenesis. In Acetogenesis pp. 63–87 Edited by Drake H.L. New York: Chapman & Hall;
    [Google Scholar]
  17. Lorowitz W.H., Bryant M.P. 1984; Peptostreptococcusproductus strain that grows rapidly with CO as the energy source. Appl Environ Microbiol 47:961–964
    [Google Scholar]
  18. Lux M.F., Drake H.L. 1992; Re-examination of the metabolic potentials of the acetogens Clostridium aceticum and Clostridium formicoaceticum: chemolithoautotrophic and aromatic-dependent growth. FEMS Microbiol Lett 95:49–56
    [Google Scholar]
  19. Lux M.F., Keith E., Hsu T., Drake H.L. 1990; Biotransformations of aromatic aldehydes by acetogenic bacteria. FEMS Microbiol Lett 67:73–78
    [Google Scholar]
  20. Matthies C., Freiberger A., Drake H.L. 1993; Fumarate dissimilation and differential reductant flow by Clostridium formicoaceticum and Clostridium aceticum . Arch Microbiol 160:273–278
    [Google Scholar]
  21. Misoph M., Drake H.L. 1996; Effect of CO2 on the fermentation capacities of the acetogen Peptostreptococcus productus U-l. J Bacteriol 178:3140–3145
    [Google Scholar]
  22. Parekh M., Keith E.S., Daniel S.L., Drake H.L. 1992; Comparative evaluation of the metabolic potentials of different strains of Peptostreptococcus productus: utilization and transformation of aromatic compounds. FEMS Microbiol Lett 94:69–74
    [Google Scholar]
  23. Ragsdale S.W. 1991; Enzymology of the acetyl-CoA pathway of CO2 fixation. Crit Rev Biochem Mol Biol 26:261–300
    [Google Scholar]
  24. Seifritz C., Daniel S.L., GöBner A., Drake H.L. 1993; Nitrate as a preferred electron sink for the acetogen Clostridium thermoaceticum . J Bacteriol 175:8008–8013
    [Google Scholar]
  25. Seitz H.-J., Schink B., Pfening N., Conrad R. 1990; Energetics of syntrophic ethanol oxidation in defined chemostat cocultures.1.Energy requirements for H2 production and H2 oxidation. Arch Microbiol 155:82–88
    [Google Scholar]
  26. Tschech A., Pfennig N. 1984; Growth yield increase linked to caffeate reduction in Acetobacterium woodii . Arch Microbiol 137:163–167
    [Google Scholar]
  27. Wood H.G., Ljungdahl L.G. 1991; Autotrophic character of acetogenic bacteria. In Variations in Autotrophic Life pp. 201–250 Edited by Shively J.M., Barton L.L. San Diego, CA: Academic Press;
    [Google Scholar]
  28. Wu Z., Daniel S.L., Drake H.L. 1988; Characterization of a CO-dependent O-demethylating enzyme system from the acetogen Clostridium thermoaceticum . J Bacteriol 170:5747–5750
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-8-1983
Loading
/content/journal/micro/10.1099/13500872-142-8-1983
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error