The Hha protein from is highly similar (82%) to the YmoA protein from Both are members of a new class of proteins that modulates gene expression, probably by influencing DNA topology. In this paper, complementation of the mutation in by the gene from has been studied. We show that the gene complements one of the phenotypic properties of mutants (high level of haemolysin production when they carry the recombinant plasmid pANN202-312) when cloned in a medium-copy-number plasmid but not when carried in a low-copy-number plasmid. Western blot analysis of the expression of YmoA in rules out inefficient expression of the protein. Surprisingly, the gene itself fails to complement the mutation when cloned in a medium-copy-number vector and causes genetic rearrangements of the chromosome as a consequence of insertion sequences mobilization.


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