1887

Abstract

Transcriptional regulation of the gene, one of the operons forming the rhamnose regulon in was studied by fusing its complete or deleted promoter to the reporter gene Analysis of β-galactosidase activities induced in these constructions grown under different conditions predicted the presence of two putative control elements: one for the RhaS regulatory protein and activating the gene not only by L-rhamnose but also by L-lyxose or L-mannose, the other for cAMP-catabolite repression protein and activating this gene in the absence of glucose. Anaerobiosis increased the promoter function two- to threefold with respect to the aerobic condition. Experiments involving complementation of strains containing the -promoter fusion and carrying a deletion in the and/or genes with plasmids bearing the rhamnose regulatory genes showed that is controlled by a regulatory cascade, in which RhaR induces and the accumulated RhaS directly activates

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/content/journal/micro/10.1099/13500872-142-7-1833
1996-07-01
2019-10-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-7-1833
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