%0 Journal Article %A Jones, Christine %A Thompson, Arthur %A England, Reg %T Guanosine 5′-diphosphate 3′-diphosphate (ppGpp), guanosine 5′-diphosphate 3′-monophosphate (ppGp) and antibiotic production in Streptomyces clavuligerus %D 1996 %J Microbiology, %V 142 %N 7 %P 1789-1795 %@ 1465-2080 %R https://doi.org/10.1099/13500872-142-7-1789 %K ppGp %K stringent response %K ppGpp %K antibiotics %K Streptomyces clavuligerus %I Microbiology Society, %X Streptomyces clavuligerus produced cephamycin C under all nutrient limitations investigated in batch culture, whilst clavulanic acid was produced under phosphate and carbon limitations. Guanosine 5′-diphosphate 3′-diphosphate (ppGpp) was produced at very low levels in all fermentations, prior to the detection of cephamycin C in the fermentation broth. Guanosine 5′-diphosphate 3′-monophosphate (ppGp) was observed at high levels in all fermentations; it appeared following a downturn in nutrient levels and immediately prior to detection of isopenicillin N synthase (IPNS). ppGp was detected following nutritional shiftdown by amino acid depletion and it was not produced via degradation of ppGpp. The results point to a possible role for ppGp in the regulation of cephamycin production in S. clavuligerus. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-7-1789