1887

Abstract

When biomass from submerged culture was filtered (100-120 μn sintered glass filter) the antibiotic yield of the retentate (mean hyphal particle diameter 103 μm) was significantly higher [1.740 mg (mg biomass)] than that of the filtrate [1.286 mg (mg biomass); mean hyphal particle diameter 88 μn]. A hypothesis to explain this is that there is a critical hyphal particle diameter, below which the particle is incapable of producing antibiotic. This would be a consequence of the site of antibiotic production occurring at a fixed distance from the growing hyphal tip. A protocol is proposed for calculation of the hypothetical critical hyphal diameter (88 μm in this case). The proportion of retentate (more productive) fraction of biomass varied between 60% and 30% during the course of a batch culture. Bioreactor stirrer speed significantly affected mean hyphal particle diameter (70 fim at 1500 r.p.m.; 124 μm at 750 r.p.m.) and antibiotic productivity [0.867 mg (mg biomass) at 1500 r.p.m.; 0.913 mg (mg biomass) at 750 r.p.m.].

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-7-1783
1996-07-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/7/mic-142-7-1783.html?itemId=/content/journal/micro/10.1099/13500872-142-7-1783&mimeType=html&fmt=ahah

References

  1. Bewick M.W., Williams S.T. 1977; Effects of addition of chloramphenicol on the growth and ultrastructure of Streptomyces venezuelae.. Microbios 19:27–35
    [Google Scholar]
  2. Bewick M. W., Williams S. T., Veitkamp C. 1977; Growth and ultrastructure of Streptomyces venezualae during chloramphenicol production.. Microbios 16:191–199
    [Google Scholar]
  3. Bushell M.E. 1988; Growth, metabolism and fermentation technology. In Biotechnology of the Actinomycetales pp. 185–215 Edited by Goodfellow M., Williams S. T. , Mordarski M. London: Academic Press;
    [Google Scholar]
  4. Campbell P.N. 1974 Statistics for Biologists Cambridge: Cambridge University Press;
    [Google Scholar]
  5. Huck T. A., Porter N., Bushell M. E. 1991; Positive selection of antibiotic-producing soil isolates. J Gen Microbiol 137:2321–2329
    [Google Scholar]
  6. i>Jadubansa P., Lethbridge G. L., Bushell M. E. 1994; Physiology of production of viable biomass and spore inoculum for the biocontrol agent Idriella (Microdochium) bolleyi.. Enzyme Microb Technol 16:1–5
    [Google Scholar]
  7. Liu Y., Yaun C., Xu M., Hu J. 1984; Differentiation of Streptomyces kanamyceticus during industrial fermentation.. Acta Microbiol Sin 24:224–229
    [Google Scholar]
  8. Lynch H.C., Bushell M. E. 1995; The physiology of erythromycin biosynthesis in cyclic fed batch culture. Microbiology 141:3105–3111
    [Google Scholar]
  9. McDermott J.F., Lethbridge G., Bushell M. E. 1993; Estimation of the kinetic constants and elucidation of trends in growth and erythromycin production in batch and continuous cultures of Saccharopolyspora erythraea using curve-fitting techniques.. Enzyme Microb Technol 15:657–663
    [Google Scholar]
  10. Nestas E., Wang D. I. C. 1983; Computer control of the penicillin fermentation using the filtration probe in conjunction with a structured process model.. Biotechnol Bioeng 25:781–796
    [Google Scholar]
  11. Nielsen J., Krabben P. 1995; Hyphal growth and fragmentation of Pénicillium chrysogenum in submerged cultures.. Biotechnol Bioeng 46:588–598
    [Google Scholar]
  12. Packer H.L., Thomas C. R. 1993; Morphological measurements on filamentous microorganisms by fully automated image analysis.. Biotechnol Bioeng 35:870–871
    [Google Scholar]
  13. Paul G. C., Kent C. A., Thomas C. R. 1994; Hyphal vacuolation and fragmentation in Pénicillium chrysogenum.. Biotechnol Bioeng 44:655–660
    [Google Scholar]
  14. Pickup K.M., Bushell M. E. 1995; Non-fragmenting variants of Streptomyces hyphae have enhanced activity of an enzyme (phospho- N-acetylmuramyl pentapeptide translocase) in peptidoglycan bio-synthesis.. J. Ferment Bioeng 79:247–251
    [Google Scholar]
  15. Pickup K. M., Nolan R. D., Bushell M. E. 1993; A method for increasing the success rate of duplicating antibiotic activity in agar and liquid culture of Streptomyces isolates in new antibiotic screens.. J Ferment Bioeng 76:89–93
    [Google Scholar]
  16. Roth M., Noack D., Reinhardt G. 1982; Properties of non-differentiating derivatives of Streptomyces hygroscopicus.. J Gen Microbiol 128:2687–2691
    [Google Scholar]
  17. Shamlou P. A., Makagiansar H. Y., Ison A. P., Lilly M. D., Thomas C. R. 1994; Turbulent breakage of filamentous microorganisms in submerged culture in mechanically stirred bioreactors.. Chem Eng Sci 49:2621–2631
    [Google Scholar]
  18. Shomura T., Yoshida J., Amani S., Kojima M., Inouye S., Niida T. 1979; Studies on Actinomycetales producing antibiotics only on agar culture. 1. Screening, taxonomy and morphology- productivity relationship of Streptomyces halstedii strain Sf -1993.. J Antibiot 32:427–435
    [Google Scholar]
  19. Trinci A. P. J. 1994; Evolution of the Quorn mycoprotein fungus Fusarium graminearum A3/5. Microbiology 140:2181–2188
    [Google Scholar]
  20. Tsuji K., Goetz J. F. 1978; High performance liquid chromatographic determination of erythromycin. J Chromatogr 147:359–367
    [Google Scholar]
  21. Williams S. T., Entwistle S., Kurylowicz W. 1974; The morphology of streptomycetes growing in media used for commercial production of antibiotics. Microbios 3:47–60
    [Google Scholar]
  22. Wilson G.C., Bushell M. E. 1995; The induction of antibiotic synthesis in Saccharopolyspora erythraea and Streptomyces hygroscopicus by growth rate down-regulation is accompanied by a down- regulation in protein synthesis rate. FEMS Microbiol Lett 129:89–96
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-7-1783
Loading
/content/journal/micro/10.1099/13500872-142-7-1783
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error